The construction of cell-density controlled three-dimensional tissues by coating micrometer-sized collagen fiber matrices on single cell surfaces
The construction of cell-density controlled three-dimensional tissues by coating micrometer-sized collagen fiber matrices on single cell surfaces
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DOI：
10.1039/c4ra09085c
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发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Akashi, Mitsuru
中科院分区：
文献类型：
--
作者：
Liu, Chun-Yen;Matsusaki, Michiya;Akashi, Mitsuru
通过利用在中性pH下胶原微纤维的形成并利用胶原纤维和整联蛋白受体之间的细胞膜相互作用，将I型胶原纤维涂覆到单细胞表面上。胶原纤维涂层厚度可控制在3 - 30 μ m。根据微米级胶原基质的厚度，成功地构建了具有可控厚度和细胞密度的多层组织。
Type I collagen fibers were coated onto single cell surfaces by capitalizing on the formation of collagen microfibers at a neutral pH and exploiting cell membrane interactions between collagen fibers and integrin receptors. Collagen fiber coat thicknesses were controllable from 3 to 30 mu m. Multi-layered tissues with controllable thicknesses and cell densities were successfully constructed depending on the thickness of micrometer-sized collagen matrices.