Rapid detection and identification of metallo-β-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis
Rapid detection and identification of metallo-β-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis
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DOI：
10.1128/jcm.01728-06
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发表时间：
2007-02-01
影响因子：
9.4
通讯作者：
Tufik, Sergio
中科院分区：
文献类型：
--
作者：
Mendes, Rodrigo E.;Kiyota, Katia A.;Tufik, Sergio
Metallo-p-lactamase enzymes (MOL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. The objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MOL-type enzymes based on the amplicon melting peak. The reference sequences of all genes encoding IMP and VIM types, SPM-1, GIM-1, and SIM-1 were downloaded from GenBank, and primers were designed to obtain amplicons showing different sizes and melting peak temperatures (T.). The real-time PCR assay was able to detect all M beta L-harboring clinical isolates, and the T-m-assigned genotypes were 100% coincident with previous sequencing results. This assay could be suitable for identification of MOL-producing gram-negative bacteria by molecular diagnostic laboratories.
