Rapid detection of enterovirus 71 by real-time TaqMan RT-PCR
Rapid detection of enterovirus 71 by real-time TaqMan RT-PCR
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DOI：
10.1016/j.jcv.2008.01.001
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发表时间：
2008-06-01
影响因子：
8.8
通讯作者：
Poh, Chit Laa
中科院分区：
文献类型：
--
作者：
Tan, Eng Lee;Yong, Li Li Gaynor;Poh, Chit Laa
背景：肠病毒71型（EV71）是手足口病（手足口病）的主要病原，并与近期在亚太地区暴发的导致死亡的神经系统并发症有关。目的：建立快速检测EV71型病毒的实时TaqMan RT-PCR方法。研究设计：基于EV71高度保守的VP1区设计特异性引物和探针。采用67例小儿疑似手足口病临床标本对实时RT-PCR的敏感性进行评价。结果：实时TaqMan RT-PCR检测EV71病毒的特异性为100%，对5个病毒拷贝具有较高的分析灵敏度。直接从临床标本中检测EV71也具有较高的灵敏度。结论：实时TaqMan RT-PCR检测临床标本中EV71病毒具有快速、灵敏的特点，可在疫情发生时更有效地采取检疫措施。(c) 2008 Elsevier B.V.版权所有
Background: Enterovirus 71 (EV71) is the main etiological agent of Hand, Foot and Mouth Disease (HFMD) and has been associated with neurological complications which resulted in fatalities during recent outbreaks in Asia Pacific region.Objective: Develop a real-time TaqMan RT-PCR for rapid detection of EV71.Study design: Specific primers and probe were designed based on highly conserved VP1 region of EV71. The sensitivity of the real-time RT-PCR was evaluated with 67 clinical specimens collected from pediatric patients with suspected HFMD.Results: Our real-time TaqMan RT-PCR showed 100% specificity in detecting EV71 and showed an analytical sensitivity of 5 viral copies. High sensitivity was also achieved in detecting EV71 directly from clinical specimens.Conclusions: Real-time TaqMan RT-PCR offers a rapid and sensitive method to detect EV71 from clinical specimens, and will allow quarantine measures to be taken more effectively during outbreaks. (c) 2008 Elsevier B.V. All rights reserved.