THE DETERMINATION OF ALBUMIN IN HUMAN BLOOD PLASMA AND SERUM - A METHOD BASED ON THE INTERACTION OF ALBUMIN WITH AN ANIONIC DYE-2-(4'-HYDROXYBENZENEAZO) BENZOIC ACID
THE DETERMINATION OF ALBUMIN IN HUMAN BLOOD PLASMA AND SERUM - A METHOD BASED ON THE INTERACTION OF ALBUMIN WITH AN ANIONIC DYE-2-(4'-HYDROXYBENZENEAZO) BENZOIC ACID
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DOI：
10.1172/jci102887
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发表时间：
1954-01-01
影响因子：
15.9
通讯作者：
BURKE, JM
中科院分区：
文献类型：
--
作者：
RUTSTEIN, DD;INGENITO, EF;BURKE, JM
建立了一种新的、简便的人血浆或血清中白蛋白的定量测定方法。该方法依赖于阴离子染料[长破折号]2-（4[图像]-羟基苯偶氮）苯甲酸[长破折号]的吸收光谱的位移，该吸收光谱是在特定条件下由血清或血浆白蛋白选择性结合染料产生的，该方法被设计用于Beckman DU型分光光度计和Evelyn光电比色计，但可以修改用于其他仪器。
A new and simple method was developed for the quantitative measurement of albumin in human blood plasma or serum. The method, dependent upon the shift in the absorption spectrum of an anionic dye[long dash]2-(4[image]-hydroxybenzeneazo) benzoic acid[long dash]produced by the selective binding of the dye by serum or plasma albumin under specified conditions, was devised for use in the Beckman model DU spectrophotometer and in the Evelyn photoelectric colorimeter, but can be modified for use with other instruments.