Enhanced photocurrent production by the synergy of hematite nanowire-arrayed photoanode and bioengineered Shewanella oneidensis MR-1
Enhanced photocurrent production by the synergy of hematite nanowire-arrayed photoanode and bioengineered Shewanella oneidensis MR-1
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通过赤铁矿纳米线阵列光阳极和生物工程希瓦氏菌 MR-1 的协同作用增强光电流产生
DOI：
10.1016/j.bios.2017.03.006
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发表时间：
2017
影响因子：
12.6
通讯作者：
He Weidong
中科院分区：
文献类型：
--
作者：
Zhu Gaolong;Yang Yun;Liu Juan;Liu Feng;Lu Anhuai;He Weidong
将半导体的光捕获能力与细菌的催化能力结合起来是提高生物电化学系统效率的一种有前途的方法。在这里，我们报道了在可见光下的太阳能辅助微生物光电化学系统（太阳能MPS）中，赤铁矿纳米线阵列光阳极和生物工程希瓦氏菌oneidensisMR-1的协同作用产生了增强的光电流。为了增加生物电子的供应，在重组体中过表达 D-乳酸转运蛋白 SO1522。 oneidensis(T-SO1522) 消化 D-乳酸的速度比野生型快 61%。 oneidenesis。在没有光照的情况下，添加野生型或重组S。 oneidensisto 该系统没有引起电流输出任何明显的增加。然而，在单太阳光照下，相对于 Ag/AgCl 和添加野生型 S，非生物对照在 0.8 V 下的光电流为 16±2 μA cm−2。 oneidensis 和重组 S. oneidensis 相对于 Ag/AgCl，在 0.8 V 电压下，光电流分别增加到 70±6 和 95±8 μA cm−2。此外，采用 T-SO1522 的太阳能 MPS 对开/关照明周期具有快速且可重复的响应，并且在 273 小时的运行中具有相对稳定的光电流产生。扫描电子显微镜（SEM）图像显示重组体和野生型赤铁矿光电极上的细胞密度相似。奥尼登西斯。这些发现揭示了复杂光催化剂-发电混合系统中代谢率对光电转换的显着影响，这对于促进用于发电和废水处理的太阳能MPS的发展具有重要意义。
Coupling the light-harvesting capabilities of semiconductors with the catalytic power of bacteria is a promising way to increase the efficiency of bioelectrochemical systems. Here, we reported the enhanced photocurrents produced by the synergy of hematite nanowire-arrayed photoanode and the bio-engineeredShewanella oneidensisMR-1 in a solar-assisted microbial photoelectrochemical system (solar MPS) under the visible light. To increase the supply of bioelectrons, theD-lactate transporter, SO1522, was overexpressed in the recombinantS. oneidensis(T-SO1522) that could digestD-lactate 61% faster than the wild-typeS. oneidenesis.Without light illumination, the addition of either the wild-type or the recombinantS. oneidensisto the system did not induce any obvious increase in the current output. However, under one-sun illumination, the photocurrent of the abiotic control was 16±2 μA cm−2at 0.8 V vs. Ag/AgCl, and the addition of the wild-typeS. oneidensisand the recombinantS. oneidensisincreased the photocurrent to 70±6 and 95±8 μA cm−2, respectively, at 0.8 V vs. Ag/AgCl. Moreover, the solar MPS with T-SO1522 presented quick and repeatable responses to the on/off illumination cycles, and had relatively stable photocurrent generation in the 273-h operation. Scanning electron microscope (SEM) images showed that the cell density on the hematite photoelectrode was similar between the recombinant and the wild-typeS. oneidensis. These findings revealed the pronounced influence of metabolic rates on the light-to-electricity conversion in the complex photocatalyst-electricigen hybrid system, which is important to promote the development of the solar MPS for electricity production and wastewater treatment.