Protein elicitor GP1pro targets aquaporin NbPIP2;4 to activate plant immunity
Protein elicitor GP1pro targets aquaporin NbPIP2;4 to activate plant immunity
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DOI：
10.1111/pce.14634
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发表时间：
2023-06-01
影响因子：
7.3
通讯作者：
Feng，Juntao
中科院分区：
文献类型：
--
作者：
Sun，Yubo;Ren，Xingyu;Feng，Juntao
新的蛋白激发子GP1pro是从卡纳链霉菌ZX01中分离和鉴定的糖蛋白GP-1的蛋白质组分。GP1pro可诱导抗病相关基因和次生代谢物的积累，产生活性氧(ROS)和过敏性反应(HR)。它最终调节植物的防御反应。进一步的分析表明，GP1Pro与植物质膜上的PIP2家族水通道蛋白NbPIP2；4相互作用。PM的定位对于诱导GP1Pro耐药是必要的。这些结果表明，NbPIP2；4作为一种H_2O_2转运体，对植物免疫和ROS积累起到积极的调节作用。综上所述，本研究阐明了GP1Pro通过靶向植物水通道蛋白NbPIP2；4和运输质外体到细胞质体内的过氧化氢来调节植物免疫，从而启动宿主细胞防御的一条保守的新途径。
The novel protein elicitor GP1pro is the protein component of glycoprotein GP‐1 isolated and identified fromStreptomyces kanasensisZX01. GP1pro induces the production of reactive oxygen species (ROS) and a hypersensitive response (HR), along with the accumulation of resistance‐related genes and secondary metabolites. It ultimately regulates plant defence responses. Further analysis revealed that GP1pro interacts with the PIP2‐family aquaporin protein NbPIP2;4 on the plant plasma membrane (PM) inNicotiana benthamiana. PM localization is necessary for inducing GP1pro resistance. These results demonstrate that NbPIP2;4 acts as a H2O2transporter to positively regulate plant immunity and ROS accumulation. In summary, this study elucidates a conserved and novel pathway caused by GP1pro to initiate host cellular defences by targeting the plant aquaporin protein NbPIP2;4 and transporting apoplast‐to‐cytoplast H2O2to regulate plant immunity.