Hypothermia attenuates protective effects of ginkgolides on astrocytes from ischemia/reperfusion injury
Hypothermia attenuates protective effects of ginkgolides on astrocytes from ischemia/reperfusion injury
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DOI：
10.1016/j.neuint.2008.12.009
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发表时间：
2009-09-01
影响因子：
4.2
通讯作者：
Ya, Ke
中科院分区：
文献类型：
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作者：
Fang, Du;Ming, Qian Zhong;Ya, Ke
低温和银杏内酯的神经保护作用已得到充分证实。我们首先研究了低温（32或28摄氏度）或银杏内酯是否对星形胶质细胞有保护作用，以防止缺血和再灌注引起的损伤。我们证明，银杏内酯，而不是低温，有一个显着的时间和浓度依赖性的保护作用，在缺血性星形胶质细胞。然后，我们研究了低温和银杏内酯联合治疗是否具有协同作用，以保护星形胶质细胞免受缺血和再灌注诱导的损伤。将细胞与18.75、37.5或75 μ g/ml银杏内酯在37.32或28 ℃下孵育24、48或72小时，然后暴露于缺血（24小时），然后再灌注（24小时）。数据显示，共同处理诱导显着下降，而不是增加，我们已经预期的，在他们的细胞活力和抗凋亡能力相比，由银杏内酯单独处理的细胞。Western blot分析表明，低温（32 ℃或28 ℃，24 h）对缺氧诱导因子-1 α（HIF-1 α）蛋白的表达没有影响，提示HIF-1 α与低温对银杏内酯的不良影响无关。这一发现意味着进一步研究低温对临床常用药物的药理作用或疗效的影响的重要性。(C)2009年由Elsevier Ltd.出版
The neuroprotective roles of both hypothermia and ginkgolides have been well confirmed. We first examined whether hypothermia (32 or 28 degrees C) or ginkgolides have a protective effect on astrocytes against ischemia and reperfusion-induced injury. We demonstrated that ginkgolides, but not hypothermia, have a significantly time- and concentration-dependent protective role in ischemic astrocytes. We then investigated whether co-treatment with hypothermia and ginkgolides has a synergistic role to protect astrocytes against ischemia and reperfusion-induced injury. Cells were incubated with 18.75, 37.5 or 75 mu g/ml of ginkgolides at 37.32 or 28 degrees C for 24,48 or 72 h before exposure to ischemia (24 h) and then reperfusion (24 h). Data showed that the co-treatment induced a significant decrease, rather than an increase as we had expected, in their cellular viabilities and anti-apoptotic abilities as compared with the cells treated by ginkgolides only. Western blot analysis demonstrated that hypothermia (32 or 28 degrees C for 24 h) has no effect on the expression of Hypoxia-inducible factor-1alpha (HIF-1alpha) protein, suggesting that HIF-1alpha is not associated with the adverse effect of hypothermia on ginkgolides. The findings imply the importance of further investigating the effects of hypothermia on the pharmacological role or therapeutic efficacy of drugs commonly used clinically. (C) 2009 Published by Elsevier Ltd.