YEAST HISTONE MESSENGER-RNA IS POLYADENYLATED
YEAST HISTONE MESSENGER-RNA IS POLYADENYLATED
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DOI：
10.1093/nar/8.23.5725
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
HEREFORD, L
中科院分区：
文献类型：
--
作者：
FAHRNER, K;YARGER, J;HEREFORD, L
Histone mRNA fromS.cerevisiaehas been identified and partially purified. The RNA is quantitatively retained on oligo (dT) cellulose or poly(U) sepharose as assayed byin vitrotranslation or hybridization of radiolabelled cloned yeast histone sequences to RNA immobilized on DBM paper. Retention of yeast histone mRNA on either of these chromatographic systems is most likely the result of polyadenylation since, when primed with oligo (dT), the RNA is an extremely good template for reverse transcriptase, as determined by hybrid arrest translation or by hybridization toD.melanogasterhistone DNA sequences.
