Endophytic taxol-producing fungi from bald cypress, Taxodium distichum
Endophytic taxol-producing fungi from bald cypress, Taxodium distichum
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DOI：
10.1099/13500872-142-8-2223
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发表时间：
1996-08-01
期刊：
影响因子：
--
通讯作者：
Ford, EJ
中科院分区：
文献类型：
--
作者：
Li, JY;Strobel, G;Ford, EJ
小孢拟盘多毛孢菌作为一系列菌株出现在秃柏、二列红豆杉中。这些生物体作为内生菌生活在树皮、韧皮部和木质部中，并且分离物在常见的实验室培养基上显示出不同的培养和显微特征。这些真菌中的许多通过部分纯化的培养提取物与针对紫杉醇的特异性单克隆抗体的反应性来确定紫杉醇。在一株小孢青霉（CP-4）的情况下，从培养基中分离出紫杉醇，并通过色谱和光谱方法显示与真正的紫杉醇相同。
Pestalotiopsis microspora occurs as a range of strains in bald cypress, Taxodium distichum. The organisms live as endophytes in the bark, phloem and xylem, and isolates show differences in cultural and microscopic characteristics on common laboratory media, Many of these fungi make taxol as determined by the reactivity of partially purified culture extracts with specific monoclonal antibodies against taxol. In the case of one strain of P. microspora (CP-4), taxol was isolated from culture medium and was shown to be identical to authentic taxol by chromatographic and spectroscopic means.