Silencing of developmental genes in Hydra
Silencing of developmental genes in Hydra
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DOI：
10.1006/dbio.1999.9407
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发表时间：
1999-10-01
影响因子：
2.7
通讯作者：
Bosch, TCG
中科院分区：
文献类型：
--
作者：
Lohmann, JU;Endl, I;Bosch, TCG
通过同源克隆或系统特异性策略，许多发育控制基因已在多种生物体中分离出来。然而，功能性基因测试仅适用于少数模式生物，特别是在许多动物中缺失，而这些动物在了解发育和基因功能的演变方面占据着关键地位。双链RNA介导的干扰（RNAi）开辟了一条在这种“非遗传”生物中进行功能研究的途径。在这里，我们表明，RNAi可以用来测试发育基因的功能，在刺胞动物水螅，发育研究的经典模型。引入对应于头部特异性基因KS1的双链RNA引起KS1转录物的强烈消耗。ks1功能丧失的息肉表现出严重的缺陷，头部形成，表明一个重要的作用，ks1水螅头的发展。我们的研究结果首次证明了水螅中有效的基因沉默。RNAi为各种功能研究提供了一个切入点，并为分析Hydra中调控基因的层次结构提供了一种直接的方法，直到现在，Hydra还不适合功能丧失遗传学。(C)北京：科学出版社.
Numerous developmental control genes have been isolated in a variety of organisms by either homology cloning or system-specific strategies. functional genetic tests, however, are available for only a few model organisms and particularly are missing in a number of animals that occupy key positions for understanding the evolution of development and gene function. Double-stranded RNA-mediated interference (RNAi) opens a way to perform functional studies in such "nongenetic" organisms. Here we show that RNAi can be used to test the function of developmental genes in the cnidarian Hydra, a classical model for developmental studies. Introduction of double-stranded RNA corresponding to the head-specific gene ks1 caused strong depletion of ks1 transcripts. ks1 loss-of-function polyps exhibited severe defects in head formation, indicating an important role of ks1 in Hydra head development. Our results demonstrate for the first time efficient gene silencing in Hydra. RNAi provides an entry point for a variety of functional studies and a direct approach for analyzing the hierarchy of regulatory genes in Hydra, which until now has not been amenable to loss-of-function genetics. (C) 1999 Academic Press.