Cytological techniques to analyze meiosis in Arabidopsis arenosa for investigating adaptation to polyploidy.
Cytological techniques to analyze meiosis in Arabidopsis arenosa for investigating adaptation to polyploidy.
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DOI：
10.3389/fpls.2013.00546
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发表时间：
2014
影响因子：
5.6
通讯作者：
Franklin FC
中科院分区：
文献类型：
--
作者：
Higgins JD;Wright KM;Bomblies K;Franklin FC
拟南芥是模式植物拟南芥的近亲，在自然界中以稳定的二倍体和同源四倍体群体存在。天然四倍体已经适应了全基因组复制，并且通常不会出现减数分裂错误，如多价和单价形成，这可能导致染色体不分离和生育能力降低。对二倍体和同源四倍体之间强烈分化的基因进行基因组扫描，发现了一个可能负责适应多倍体减数分裂的减数分裂基因子集。为了研究沙棘适应多倍体状态的机制，以及鉴定出的潜在适应性多态性的功能，需要进行彻底的细胞学分析。因此，在本章中，我们描述了分析羊草雄性减数分裂的方法和技术，包括最佳植物生长条件，免疫细胞学和细胞学方法，这些方法是为了了解同源四倍体的减数分裂适应性而开发的。此外，我们还提出了减数分裂细胞学图谱，作为特定阶段的参考，并讨论了从二倍体和同源四倍体之间的减数分裂比较中产生的观察结果。
Arabidopsis arenosa is a close relative of the model plant A. thaliana, and exists in nature as stable diploid and autotetraploid populations. Natural tetraploids have adapted to whole genome duplication and do not commonly show meiotic errors such as multivalent and univalent formation, which can lead to chromosome non-disjunction and reduced fertility. A genome scan for genes strongly differentiated between diploid and autotetraploid A. arenosa identified a subset of meiotic genes that may be responsible for adaptation to polyploid meiosis. To investigate the mechanisms by which A. arenosa adapted to its polyploid state, and the functionality of the identified potentially adaptive polymorphisms, a thorough cytological analysis is required. Therefore, in this chapter we describe methods and techniques to analyze male meiosis in A. arenosa, including optimum plant growth conditions, and immunocytological and cytological approaches developed with the specific purpose of understanding meiotic adaptation in an autotetraploid. In addition we present a meiotic cytological atlas to be used as a reference for particular stages and discuss observations arising from a comparison of meiosis between diploid and autotetraploid A. arenosa.
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影响因子：
2.6
作者：
Comai, L;Tyagi, AP;Lysak, MA
通讯作者：
Lysak, MA
影响因子：
2.6
作者：
Moran, ES;Armstrong, SJ;Jones, GH
通讯作者：
Jones, GH
DOI：
10.1199/tab.0001
发表时间：
2002-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Al-Shehbaz, Ihsan A;O'Kane, Steve L Jr
通讯作者：
O'Kane, Steve L Jr
影响因子：
--
作者：
Carvalho, Ana;Delgado, Margarida;Neves, Nuno
通讯作者：
Neves, Nuno
影响因子：
10.5
作者：
Sanchez-Moran, Eugenio;Santos, Juan-Luis;Franklin, F. Christopher H.
通讯作者：
Franklin, F. Christopher H.