Feasibility of urinary microRNA profiling detection in intrahepatic cholestasis of pregnancy and its potential as a non-invasive biomarker.
Feasibility of urinary microRNA profiling detection in intrahepatic cholestasis of pregnancy and its potential as a non-invasive biomarker.
复制标题
尿液 microRNA 谱检测妊娠期肝内胆汁淤积症的可行性及其作为非侵入性生物标志物的潜力
DOI：
10.1038/srep31535
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发表时间：
2016-08-18
影响因子：
4.6
通讯作者：
Ding M
中科院分区：
文献类型：
--
作者：
Ma L;Zhang XQ;Zhou DX;Cui Y;Deng LL;Yang T;Shao Y;Ding M
妊娠期肝内胆汁淤积症（ICP）是一种妊娠相关性肝病，可导致母儿并发症。循环microRNA（miRNAs）已成为许多疾病的候选生物标志物。到目前为止，尚未研究ICP的循环miRNA谱。为了评估尿miRNA作为ICP的非侵入性生物标志物，首先通过个体定量逆转录酶聚合酶链反应（qRT-PCR）分析来自筛选组（包括10名ICP和10名健康妊娠）的尿样品中的差异miRNA谱。然后使用单独的qRT-PCR测定通过具有40个ICP和50个健康妊娠的验证集来验证所选择的候选miRNA。ICP患者尿液中hsa-miR-151- 3 p和hsa-miR-300的表达水平较正常孕妇尿液中的表达水平显著下调，hsa-miR-671- 3 p和hsa-miR-369- 5 p的表达水平显著上调（p < 0.05，假发现率< 0.05）。使用四种miRNA构建二元逻辑回归模型。受试者工作特征曲线下面积为0.913（95%置信区间= 0.847至0.980;灵敏度= 82.9%，特异性= 87.0%）。因此，ICP患者尿液microRNA谱检测是可行的，孕妇尿液microRNA有可能成为ICP诊断的非侵入性生物标志物。
Intrahepatic cholestasis of pregnancy (ICP), a pregnancy-related liver disease, leads to complications for both mother and fetus. Circulating microRNAs (miRNAs) have emerged as candidate biomarkers for many diseases. So far, the circulating miRNAs profiling of ICP has not been investigated. To assess the urinary miRNAs as non-invasive biomarkers for ICP, a differential miRNA profiling was initially analyzed by individual quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assay in urinary samples from a screening set including 10 ICP and 10 healthy pregnancies. The selected candidate miRNAs were then validated by a validation set with 40 ICP and 50 healthy pregnancies using individual qRT-PCR assay. Compared with the expression in urine of healthy pregnant women, the expression levels of hsa-miR-151-3p and hsa-miR-300 were significantly down-regulated, whereas hsa-miR-671-3p and hsa-miR-369-5p were significantly up-regulated in urine from ICP patients (p < 0.05 and false discovery rate < 0.05). A binary logistic regression model was constructed using the four miRNAs. The area under the receiver operating characteristic curve was 0.913 (95% confidence interval = 0.847 to 0.980; sensitivity = 82.9%, specificity = 87.0%). Therefore, urinary microRNA profiling detection in ICP is feasible and maternal urinary miRNAs have the potential to be non-invasive biomarkers for the diagnosis of ICP.