Both hypersensitive and non-hypersensitive responses are associated with resistance in Salix viminalis against the gall midge Dasineura marginemtorquens
Both hypersensitive and non-hypersensitive responses are associated with resistance in Salix viminalis against the gall midge Dasineura marginemtorquens
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DOI：
10.1093/jxb/eri318
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发表时间：
2005-12-01
影响因子：
6.9
通讯作者：
Wingsle, G
中科院分区：
文献类型：
--
作者：
Höglund, S;Larsson, S;Wingsle, G
过敏性反应(HR)在植物对病原菌的抗性中起着重要作用。虽然几乎没有明确的文献，但人们经常声称HR在植物对昆虫的抗性中也很重要。在青柳中发现了较大的抗性遗传变异。本文报道了一个全同胞家系的幼虫表现和诱导反应的变异：36个同胞株是完全抗性的(幼虫在卵孵化后48h内死亡，没有虫害诱导)，11个株是完全感病的，25个株是可变的(活的和死的幼虫在同一株上存在)。抗性与HR有关，但程度不同；21个完全抗性的基因型表现出典型的HR症状(许多明显的坏死点)，而其余15个基因型没有或很少出现这种症状。作为HR标志的过氧化氢在表现HR症状的基因中被诱导，而在无症状的抗性基因和敏感基因中不被诱导。这些数据表明，过氧化氢的产生和伴随的细胞死亡，不能解释无症状反应中幼虫的死亡。可能存在另一种尚不清楚的抗药性机制。如果是这样，那么这种未知的机制可能也有助于表现HR的植物的抗性。在这种相互作用中观察到的明显的复杂性，以及与对适应的昆虫物种的抗性相关的可见和不可见的植物反应，可能对研究其他植物-昆虫相互作用中的抗性因素具有重要意义。
Hypersensitivity responses (HR) play a major role in plant resistance to pathogens. It is often claimed that HR is also important in plant resistance to insects, although there is little unambiguous documentation. Large genotypic variation in resistance against the gall midge Dasineura marginemtorquens is found in Salix viminalis. Variation in larval performance and induced responses within a full-sib S. viminalis family is reported here; 36 sibling plants were completely resistant (larvae died within 48 h after egg hatch, no gall induction), 11 plants were totally susceptible, 25 plants were variable (living and dead larvae present on the same plant). Resistance was associated with HR, but to different degrees; 21 totally resistant genotypes showed typical HR symptoms (many distinct necrotic spots) whereas the remaining 15 genotypes showed no, or very few, such symptoms. Hydrogen peroxide, used as a marker for HR, was induced in genotypes expressing HR symptoms but not in resistant genotypes without symptoms, or in susceptible genotypes. These data suggest that production of hydrogen peroxide, and accompanying cell death, cannot explain larval mortality in the symptomless reaction. Another, as yet unknown, mechanism of resistance may be present. If so, then it is possible that this unknown mechanism also contributes to resistance in plants displaying HR. The apparent complexity observed in this interaction, with both visible and invisible plant responses associated with resistance against an adapted insect species, may have implications for the study of resistance factors in other plant-insect interactions.