Serum- and glucocorticoid-inducible kinases in microglia.
Serum- and glucocorticoid-inducible kinases in microglia.
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DOI：
10.1016/j.bbrc.2016.07.094
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发表时间：
2016-09-09
影响因子：
3.1
通讯作者：
Ueki T
中科院分区：
文献类型：
--
作者：
Inoue K;Sakuma E;Morimoto H;Asai H;Koide Y;Leng T;Wada I;Xiong ZG;Ueki T
小胶质细胞来源于骨髓细胞，参与中枢神经系统的免疫和炎症反应。它们不仅在感染性疾病和中风后的炎症中起重要作用，而且在精神分裂症等精神疾病中也起重要作用。虽然最近的研究表明血清和糖皮质激素诱导的激酶（SGKs）在其他免疫细胞如巨噬细胞，T细胞和树突状细胞中的重要性，但它们在小胶质细胞中的作用仍然未知。在这里，我们第一次报道了SGK 1和SGK 3在多种小胶质细胞系中表达。SGK抑制剂gsk 650394抑制细胞活力。此外，脂多糖诱导的炎症调节因子iNOS和TNFα的表达被gsk 650394增强。此外，gsk 650394还能促进NF-κB的转位。总之，这些发现表明SGKs可能在调节小胶质细胞活力和炎症反应中起重要作用。
Microglia are derived from myelogenous cells and contribute to immunological and inflammatory responses in central nervous system. They play important roles not only in infectious diseases and inflammation after stroke, but also in psychiatric diseases such as schizophrenia. While recent studies suggest the significances of serum- and glucocorticoid-inducible kinases (SGKs) in other immune cells such as macrophages, T cells and dendritic cells, their role in microglia remains unknown. Here we, for the first time, report that SGK1 and SGK3 are expressed in multiple microglial cell lines. An SGK inhibitor, gsk650394, inhibits cell viability. In addition, lipopolysaccharide-induced expression of inflammatory regulators iNOS and TNFα was enhanced by gsk650394. Furthermore, translocation of NF-κB was enhanced by gsk650394. Taken together, these findings suggest that SGKs may play an important role in regulating microglial viability and inflammatory responses.