Exploiting the dynamics of S-phase tracers in developing brain: Interkinetic nuclear migration for cells entering versus leaving the S-phase
Exploiting the dynamics of S-phase tracers in developing brain: Interkinetic nuclear migration for cells entering versus leaving the S-phase
复制标题
DOI：
10.1159/000017426
复制
发表时间：
2000-01-01
影响因子：
2.9
通讯作者：
Nowakowski, RS
中科院分区：
文献类型：
--
作者：
Hayes, NL;Nowakowski, RS
用双标记技术比较了在胚胎第14天(E14)发育中的小鼠皮质中两种用于体内研究中枢神经系统发育中细胞增殖的S时相标记物--氚胸苷(H-3-TdR)和溴脱氧尿苷(BUDR)。这两种示踪剂的标记效率和可检测性大致相当，没有证据表明示踪剂之间存在显著的相互作用，这取决于给药顺序。对于这两种示踪剂，标记S相细胞到可检测所需的加载时间估计为
Two S-phase markers for in vivo studies of cell proliferation in the developing central nervous system, tritiated thymidine (H-3-TdR) and bromodeoxyuridine (BUdR), were compared using double-labeling techniques in the developing mouse cortex at embryonic day 14 (E14). The labeling efficiencies and detectability of the two tracers were approximately equivalent, and there was no evidence of significant tracer interactions that depend on order of administration. For both tracers, the loading time needed to label an S-phase cell to detectability is estimated at