Isolation and Molecular Characterization of Entamoeba nuttalli Strains Showing Novel Isoenzyme Patterns from Wild Toque Macaques in Sri Lanka
Isolation and Molecular Characterization of Entamoeba nuttalli Strains Showing Novel Isoenzyme Patterns from Wild Toque Macaques in Sri Lanka
复制标题
DOI：
10.1111/jeu.12265
复制
发表时间：
2016-03
影响因子：
2.2
通讯作者：
H. Tachibana;T. Yanagi;M. Feng;K. Bandara;Seiki Kobayashi;Xunjia Cheng;K. Hirayama;R. Rajapakse-R.-Rajapa
中科院分区：
文献类型：
--
作者：
H. Tachibana;T. Yanagi;M. Feng;K. Bandara;Seiki Kobayashi;Xunjia Cheng;K. Hirayama;R. Rajapakse-R.-Rajapa
我们建议将一种来自恒河猴的强毒阿米巴菌株P19 - 061405重新命名为纳氏内阿米巴，并将其在遗传学上定位于E. histolytica和E.差异例如E. nuttalli最初被描述为在斯里兰卡的托克猕猴中发现的阿米巴，对野生托克猕猴中内阿米巴属物种的流行和特征进行了检查。对6个地区的227份粪便标本进行PCR检测，结果显示E. nuttalli、刺尾草E. dispar和E.溶组织性分别为18.5%、0.4%和0%。十五E。从五个地点成功培养了纳塔利菌株。与P19 - 061405株相比，18 S核糖体RNA基因仅显示3个核苷酸差异。在同工酶分析中，斯里兰卡菌株中的己糖激酶模式与P19 - 061405菌株中的己糖激酶模式不同，并且通过基因分析证实了差异。用斯里兰卡E.仓鼠中的nuttalli菌株导致阿米巴脓肿形成和体重减轻。这些结果表明，E. nuttalli在野生的toque猕猴中普遍存在，并且该品系的几个特征是独特的。我们的结论是，使用名称E。nuttalli适合于在非人类灵长类动物中发现的新内阿米巴属物种。
We have proposed the revival of the name Entamoeba nuttalli for a virulent ameba strain, P19‐061405, from a rhesus macaque and located it phylogenetically between E. histolytica and E. dispar. As E. nuttalli was originally described for an ameba found in a toque macaque in Sri Lanka, the prevalence and characteristics of Entamoeba species in wild toque macaques were examined. PCR analysis of 227 stool samples from six locations showed positive rates for E. nuttalli, E. dispar, and E. histolytica of 18.5%, 0.4%, and 0%, respectively. Fifteen E. nuttalli strains were cultured successfully from five locations. The 18S ribosomal RNA gene showed only three nucleotide differences in comparison with P19‐061405 strain. In isoenzyme analysis, the pattern of hexokinase in Sri Lankan strains was different from that of P19‐061405 strains and the difference was confirmed by analysis of the genes. Hepatic inoculation of one of the Sri Lankan E. nuttalli strains in hamsters resulted in amebic abscess formation and body weight loss. These results demonstrate that E. nuttalli is prevalent in wild toque macaques and that several characteristics of the strains are unique. We conclude that use of the name E. nuttalli is appropriate for the new Entamoeba species found in nonhuman primates.