Prevalence and characterization of Salmonella enterica serovar Weltevreden from imported seafood
Prevalence and characterization of Salmonella enterica serovar Weltevreden from imported seafood
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DOI：
10.1016/j.fm.2007.09.001
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发表时间：
2008-02-01
影响因子：
5.3
通讯作者：
Cerniglia, Carl E.
中科院分区：
文献类型：
--
作者：
Ponce, Elizabeth;Khan, Ashraf A.;Cerniglia, Carl E.
2001-2005年，从美国进口海产品中分离出肠炎沙诺菌210株。在分离的64种不同的血清型中，以大肠杆菌血清型welteveden菌株最多。采用脉冲场凝胶电泳（PFGE）、质粒谱和抗生素敏感性对37株Weltevreden Sahnonella菌株进行了遗传多样性鉴定。我们的结果显示，抗生素耐药的频率很低；37株中有35株对氨苄西林、四环素、氯霉素、庆大霉素、磺胺恶唑、链霉素和卡那霉素敏感。只有来自菲律宾和印度的两个分离株分别对氨苄西林和四环素以及链霉素、磺胺恶唑和四环素表现出耐药性。在37株分离株中，2株不携带任何质粒，35株携带多个小质粒和大质粒。根据质粒谱将这些分离菌株分为10种不同的类型。获得4个不同的PFGE聚类，遗传相似性为66 ~ 76%。4组分离物（每组由2个或3个分离物组成）在PFGE图谱上显示100%的相似性。其中一组包括2003年、2004年和2005年在越南从鱼和虾中分离出来的菌株。其他菌株包括2000年、2004年和2005年在越南、印度尼西亚和泰国分离出的螺、虾和鱼。我们的研究结果表明，在最近监测的进口海产品中，肠道沙门氏菌血清型weltevden具有遗传多样性和时间持久性。Elsevier Ltd.出版。
During 2001-2005, 2 10 Sahnonella enterica strains were isolated from seafood samples imported into US. Strains of S. enterica serovar Weltevreden were the most predominantly found among the 64 different serovars isolated. A total of 37 Sahnonella Weltevreden isolates were characterized by pulsed-field gel clectrophoresis (PFGE), plasmid profiles and antibiotic susceptibility to assess genetic diversity. Our results showed a low frequency of antibiotic resistance; 35 of the 37 isolates were sensitive to ampicillin, tetracycline, chloramphenicol, gentamicin, sulfisoxazole, streptomycin and kanamycin. Only two isolates, from samples originating in the Philippines and India, showed resistance to ampicillin and tetracycline and to streptomycin, sulfisoxazole and tetracycline, respectively. Of the 37 isolates, two isolates did not carry any plasmid and 35 isolates harbored several small and mega-plasmids. These isolates were differentiated into 10 distinct types based on plasmid profiles. Four different PFGE clusters were obtained with a genetic similarity of 66-76%. Four groups of isolates (formed by two or three isolates each) showed 100% similarity in the PFGE profiles. One of these groups included strains isolated in Vietnam in 2003, 2004 and 2005 from fish and shrimp. The other groups included strains isolated in Vietnam, Indonesia and Thailand in 2000, 2004 and 2005 front snail, shrimp and fish. Our findings show genetic diversity and temporal persistence of S. enterica serovar Weltevreden in recently monitored seafood imports. Published by Elsevier Ltd.