Chicken cyclophilin A is an inhibitory factor to influenza virus replication.
Chicken cyclophilin A is an inhibitory factor to influenza virus replication.
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鸡亲环蛋白A是流感病毒复制的抑制因子
DOI：
10.1186/1743-422x-7-372
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发表时间：
2010-12-30
期刊：
影响因子：
4.8
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Xu C;Meng S;Liu X;Sun L;Liu W
背景提高家禽对流感的抵抗力在科学和经济上的重要性是显而易见的。鸡对流感病毒非常敏感。据报道，人细胞亲环素A（CypA）在293 T细胞中削弱流感病毒感染。鸡CypA（chCypA）是否抑制流感病毒复制尚不清楚。鸡抗流感病毒的分子机制尚待研究。该基因的开放阅读框（ORF）为498 bp，编码165个氨基酸，分子量约为17.8 kDa，与哺乳动物CypA基因高度同源。chCypA显示出预期的抗流感活性。ChCypA蛋白被证明能够与流感病毒M1蛋白特异性相互作用。CEF细胞中chCypA的过表达降低了细胞对流感病毒的易感性。与不表达chCypA的细胞相比，在表达chCypA的细胞中重组流感病毒A/WSN/33的产量降低至三分之一。ChCypA广泛分布于鸡的多种组织中。它定位于鸡胚成纤维细胞（CEF）的细胞质中。禽流感病毒感染可引起其从细胞质向细胞核转位。禽流感病毒感染对ChCypA的表达没有显著上调。本研究表明，chCypA是一种抑制流感病毒复制的蛋白质，作为一种内在的免疫因子对流感病毒infection.ConclusionThe目前的数据表明，chCypA具有抗流感病毒的活性，可以考虑遗传改良，以抵抗流感病毒在鸡。
BackgroundThe importance of enhancing influenza resistance in domestic flocks is quite clear both scientifically and economically. Chicken is very susceptible to influenza virus. It has been reported that human cellular cyclophilin A (CypA) impaired influenza virus infection in 293T cells. Whether chicken CypA (chCypA) inhibits influenza virus replication is not known. The molecular mechanism of resistance in chicken to influenza virus remains to be studied.ResultsThe chCypA gene was isolated and characterized in the present study. It contained an ORF of 498 bp encoding a polypeptide of 165 amino acids with an estimated molecular mass of 17.8 kDa sharing high identity with mammalian CypA genes. The chCypA demonstrated an anti-influenza activity as expected. ChCypA protein was shown to be able to specifically interact with influenza virus M1 protein. Cell susceptibility to influenza virus was reduced by over-expression of chCypA in CEF cells. The production of recombinant influenza virus A/WSN/33 reduced to one third in chCypA expressing cells comparing to chCypA absent cells. ChCypA was widely distributed in a variety of chicken tissues. It localized in cytoplasm of chicken embryo fibroblast (CEF) cells. Avian influenza virus infection induced its translocation from cytoplasm into nucleus. ChCypA expression was not significantly up-regulated by avian influenza virus infection. The present study indicated that chCypA was an inhibitory protein to influenza virus replication, suggesting a role as an intrinsic immunity factor against influenza virus infection.ConclusionThe present data demonstrates that chCypA possesses anti-influenza virus activity which allows the consideration of genetic improvement for resistance to influenza virus in chickens.