The role of alkylphosphocholines in retinal Muller glial cell proliferation
The role of alkylphosphocholines in retinal Muller glial cell proliferation
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DOI：
10.1080/02713680801956460
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发表时间：
2008-04-01
影响因子：
2
通讯作者：
Eichler, Wolfram
中科院分区：
文献类型：
--
作者：
Eibl, Kirsten H.;Schwabe, Kerstin;Eichler, Wolfram
目的：研究烷基磷胆碱(APC)对体外培养的Muller胶质细胞增殖和F-肌动蛋白应激纤维分布的影响。材料和方法：用APC(C18：1-PC和C22：1-PC)+/-胎牛血清培养Muller细胞。BrdU标记和四氮唑蓝还原比色法检测细胞增殖。用台盼蓝排除法测定其毒性。用FITC-鬼臼蛋白染色检测F-肌动蛋白应激纤维的分布。结果：APC能有效抑制人和大鼠Muller神经胶质细胞的增殖和缺氧诱导的细胞上调。F-肌动蛋白应激纤维的体外无毒浓度。结论：APC可预防视网膜脱离后血清刺激和低氧所致的视网膜内改变。
Purpose: Alkylphosphocholines (APCs) are investigated for their effect on Muller glial cell proliferation and F-actin stress fiber distribution in vitro. Materials and Methods: Muller cells were incubated with APCs (C18:1-PC and C22:1-PC) +/- fetal calf serum. Proliferation was assessed by BrdU labeling and with the tetrazolium dye-reduction assay. Toxicity was measured using the trypan blue exclusion assay. The distribution of F-actin stress fibers was determined using FITC-phalloidin staining. Results: APCs are effective inhibitors of human and rat Muller glial c ell proliferation and hypoxia-induced up-regulation. of F-actin stress fibers in vitro in non-toxic concentrations. Conclusions: APCs might prevent intraretinal changes as a result of serum stimulation and hypoxia following retinal detachment.