Fluorometric Measurement of Callose and Other 1,3-β-Glucans
Fluorometric Measurement of Callose and Other 1,3-β-Glucans
复制标题
胼胝质和其他 1,3-β-葡聚糖的荧光测量
DOI：
10.1007/978-3-642-83349-6_7
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
H. Kauss
中科院分区：
文献类型：
--
作者：
H. Kauss
胼胝质一词起源于细胞化学观察。在过去的一百年里，已经有许多例子被报道，其中染色和荧光技术揭示了某些细胞壁对位似乎是由与通常构成高等植物细胞壁的物质不同的物质组成的(有关早期显微镜工作的回顾，请参见Eschrich 1956)。后来发现这些染色性质是由于基本上线性的1，3-葡聚糖的存在(引文见Fincher和Stone 1981；Evans等人)。1984年)，尽管可能存在一些其他的联系(欣克和克拉克1982)。现在使用的术语是线性1，3-p-葡聚糖的同义词，其聚合度高到足以使聚合物不溶于水。在正常的发育过程中，如花粉管的伸长，或在花粉成熟的过程中，可能会发生茧质沉积。更令人惊叹的是，在各种压力情况下，往往相当迅速和精确的局部痂形成被认为是封闭细胞和组织的。例如，连接相邻细胞的原生质体的连接的关闭(如质膜或筛孔)，真菌菌丝试图入侵宿主细胞的部位形成乳突，以及拒绝自交不亲和的花粉(引文见Eschrich 1956；Fincher和Stone 1981；Kauss 1985,1987)。
The term callose originates from cytochemical observations. Over the last hundred years many examples have been reported where staining and fluorescence techniques reveal that certain cell-wall appositions appear to consist of substances distinct from the materials which usually constitute the cell walls of higher plants (for a review on the early microscopical work see Eschrich 1956). Later it was shown that these staining properties are due to the presence of essentially linear 1, 3-p-glucans (for citations see Fincher and Stone 1981; Evans et al. 1984), although some other linkages may be present (Hinch and Clarke 1982). The term callose is now used synonymously for linear 1, 3-p-glucans exhibiting a degree of polymerization high enough to render the polymer water-insoluble. Callose deposition may occur in the course of regular developmental steps, such as the elongation of pollen tubes, or during pollen maturation. More spectacular is an often rather rapid and precisely localized callose formation which is believed to seal cells and tissues after stress situations of various kinds. Examples are the closing of connections that unite protoplasts of contiguous cells (such as plasmadesmata or sieve pores), the formation of papillae at the sites of attempted invasion of host cells by fungal hyphae, and the rejection of self-incompatible pollen (for citations see Eschrich 1956; Fincher and Stone 1981; Kauss 1985, 1987).