IdeR is required for iron homeostasis and virulence in Mycobacterium tuberculosis.
IdeR is required for iron homeostasis and virulence in Mycobacterium tuberculosis.
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DOI：
10.1111/mmi.12441
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发表时间：
2014-01
影响因子：
3.6
通讯作者：
Rodriguez GM
中科院分区：
文献类型：
--
作者：
Pandey R;Rodriguez GM
Iron is an essential but potentially harmful nutrient, poorly soluble in aerobic conditions, and not-freely available in the human host. To acquire iron, bacteria have evolved high affinity iron acquisition systems that are expressed under iron limitation often in conjunction with virulence determinants. Because excess iron can be dangerous, intracellular iron must be tightly controlled. In mycobacteria, IdeR functions as a global iron dependent transcriptional regulator, but because inactivation of ideR is lethal for Mycobacterium tuberculosis, it has not been possible to use genetics to fully characterize this protein’s function or examine the requirement of iron regulation during tuberculosis infection. In this work, a conditional M. tuberculosis ideR mutant was generated and used to study the basis of IdeR’s essentiality. This investigation uncovered positive regulation of iron storage as a critical aspect of IdeR’s function in regular culture and a prominent factor for survival under stresses associated with life in macrophages. Moreover, this study demonstrates that IdeR is indispensable in the mouse model of tuberculosis, thereby linking iron homeostasis to virulence in M. tuberculosis.
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影响因子：
3.6
作者：
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通讯作者：
Smith, I
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15.3
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Lee WL;Gold B;Darby C;Brot N;Jiang X;de Carvalho LP;Wellner D;St John G;Jacobs WR Jr;Nathan C
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