Solid-Phase Microextraction of Human Plasma Samples for Determination of Sufentanil by Gas Chromatography–Mass Spectrometry
Solid-Phase Microextraction of Human Plasma Samples for Determination of Sufentanil by Gas Chromatography–Mass Spectrometry
复制标题
DOI：
10.1097/00007691-200212000-00014
复制
发表时间：
2002-12
影响因子：
2.5
通讯作者：
C. Paradis;C. Dufresne;M. Bolon;R. Boulieu
中科院分区：
文献类型：
--
作者：
C. Paradis;C. Dufresne;M. Bolon;R. Boulieu
建立了固相微萃取（SPME）气相色谱-质谱（GC-MS）法定量分析血浆中舒芬太尼的方法。采用浸没式固相微萃取技术对血浆中舒芬太尼的提取进行了优化。考察了样品的pH值和离子强度对固相微萃取纤维萃取分析物的影响。在碱性条件下，用65-m聚二甲基硅氧烷-二乙烯基苯（PDMS-DVB）纤维用盐析剂从血浆中萃取舒芬太尼和芬太尼（内标）30分钟。校准曲线在6-50 ng/mL浓度范围内呈线性。日内和日间相对标准偏差分别为3.6%和10.6%。对于1 mL血浆体积，定量限为6 ng/mL。考虑到选择性、简单性和低成本，所描述的SPME方法可用于从人血浆中快速提取舒芬太尼。
A solid-phase microextraction (SPME) method has been developed for the quantitative analysis of sufentanil from human plasma by gas chromatography–mass spectrometry (GC-MS). The immersion SPME sampling technique was optimized for the extraction of sufentanil from plasma. The influence of the pH and the ionic strength of the sample on the extraction of the analytes by the SPME fiber were investigated. Sufentanil and fentanyl (internal standard) were extracted from plasma with a 65-m polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber for 30 minutes using salting out agents in basic conditions. The calibration curve was linear over a concentration range of 6–50 ng/mL. Intraday and interday relative standard deviations were 3.6% and 10.6%, respectively. The limit of quantification was 6 ng/mL for a plasma volume of 1 mL. With regard to selectivity, simplicity, and low cost, the SPME method described should be useful for a rapid extraction of sufentanil from human plasma.