Evidence that Lyb-2 is critical to specific activation of B cells before they become responsive to T cell and other signals.
Evidence that Lyb-2 is critical to specific activation of B cells before they become responsive to T cell and other signals.
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证据表明LYB-2在B细胞对T细胞和其他信号响应之前对特异性激活至关重要。
DOI：
10.1084/jem.155.5.1309
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发表时间：
1982-05-01
影响因子：
15.3
通讯作者：
Boyse, E A
中科院分区：
文献类型：
--
作者：
Yakura, H;Shen, F W;Bourcet, E;Boyse, E A
Lyb-2抗体在体外可减少T依赖性抗原而非T非依赖性抗原的空斑形成细胞（PFC）的生成。单克隆Lyb-2抗体，添加到Mishell-Dutton文化的第一个2天内，但不是以后，大大减少了产生的α-绵羊红细胞（SRBC）PFC从T-耗尽的脾细胞是否提供帮助的形式，完整的T细胞或作为可溶性因子包含在混合淋巴细胞培养（MLC）上清液。在MLC和巨噬细胞（P388 D.1细胞）上清液中的可溶性因子的辅助下，LyB-2抗体同样减少了从纯化的B细胞生成α-SRBC PFC。最初的2天期间，在此期间，培养物对Lyb-2抗体减少PFC生成的敏感性逐渐降低，不受添加此类可溶性因子的时间的影响。因此，Lyb-2细胞表面分子显然不作为这些分化信号的受体。Lyb-2抗体对PFC生成的减少具有抗原依赖性，因为对一种抗原（SRBC）的PFC反应的减少不会影响对来自同一细胞群的第二种抗原（马红细胞）的PFC的后续生成。这些发现雅阁这样的观点，即LyB-2分子参与B细胞分化阶段，可能是增殖阶段，该阶段开始于抗原结合，并且在B细胞变得完全接受来自T细胞和其他细胞的信号的阶段之前。
The generation of plaque-forming cells (PFC) to T-dependent antigen, but not to T-independent antigen, is reduced in vitro by Lyb-2 antibody. Monoclonal Lyb-2 antibody, added to Mishell-Dutton cultures within the first 2 d, but not later, greatly reduces the generation of alpha-sheep erythrocyte (SRBC) PFC from T-depleted spleen cells whether help is provided in the form of intact T cells or as soluble factors contained in mixed lymphocyte culture (MLC) supernatants. Generation of alpha-SRBC PFC from purified B cells, assisted by soluble factors in MLC and macrophage (P388D.1 cell) supernatants, is similarly reduced by Lyb-2 antibody. The initial 2-d period, during which cultures are diminishingly sensitive to reduction of PFC generation by Lyb-2 antibody, is not affected by the time at which such soluble factors are added. Thus, Lyb-2 cell surface molecules evidently do not function as receptors for these differentiative signals. Reduction of PFC generation by Lyb-2 antibody is antigen dependent in the sense that reduction of the PFC response to one antigen (SRBC) does not affect subsequent generation of PFC to a second antigen (horse erythrocytes) from the same cell population. These findings accord with the view that the Lyb-2 molecule participates in a B cell differentiative phase, probably proliferative, which begins with binding of antigen and precedes the phase in which B cells become fully receptive to signals from T and other cells.