Use of coated nano zinc oxide as an additive to improve the zinc excretion and intestinal morphology of growing pigs
Use of coated nano zinc oxide as an additive to improve the zinc excretion and intestinal morphology of growing pigs
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DOI：
10.1093/jas/skz065
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发表时间：
2019-04-01
影响因子：
3.3
通讯作者：
Yin, Yu L.
中科院分区：
文献类型：
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作者：
Bai, Miaomiao M.;Liu, Hongnan N.;Yin, Yu L.
设计了两个试验，以探讨包被氧化锌纳米颗粒(NZO)对生长猪的腹泻率、抗氧化能力、肠道形态和锌排泄的影响。在试验1中，270头生长猪(初始体重21.88+/-0.8 kg)被分成3个处理，每个处理30d：(1)对照组(CG)，基础日粮中添加无锌预混料+100 mg/kg硫酸锌；(2)高锌组，基础日粮中添加无锌预混料+2,250 mg/kg氧化锌；(3)包被纳米氧化锌(CNZO)，基础日粮中添加无锌预混料+100 mg锌/kg包被亚硝酸锌。在试验2中，21头杂交生长猪(初始体重17.04+/-0.01 kg)被分成3个处理，每个处理28d：(I)HZN，基础日粮中添加无锌预混料+2,250 mg/kg的氧化锌；(Ii)低浓度的纳米氧化锌(LNZO)，基础日粮中添加5%包被的硝酸锌材料中的无锌预混料+100 mg/kg锌；(Iii)高浓度的纳米氧化锌(HNZO)，基础日粮中包含无锌预混料+100 mg/kg的包覆锌氮材料。在实验中1、与对照组相比，CNZO显著降低了腹泻率(P<；0.05)，提高了谷胱甘肽过氧化物酶和超氧化物歧化酶的活性(P<；0.05)。与HZN相比，CNZO能降低血清丙氨酸氨基转移酶、碱性磷酸酶活性和粪锌浓度(P<；0.05)。在实验中2.与HZN组相比，LNZO组和HNZO组仔猪第14天的最终体重、平均日重和腹泻率显著增加，血浆、肝脏和粪便中的锌水平显著降低(P<；0.05)。HZN组十二指肠绒毛高度和绒毛高度/隐窝深度比值高于HNZO组(P<；0.05)，而LNZO组空肠绒毛高度高于HNZO组(P<；0.05)。我们发现，CNZO(100 mg/kg锌)能提高机体的抗氧化能力，减少粪便中锌的排放。然而，与补充HNZO相比，腹泻率并没有得到有效的抑制。此外，浓度为5%的包被NZO材料对改善肠道绒毛形态更为有效。
Two experiments were designed to explore the effects of coated zinc (Zn) oxide nano-particles (NZO) on the diarrhea ratio, antioxidant capacity, intestinal morphology, and zinc excretion in growing pigs. In Exp.1, 270 growing pigs (21.88 +/- 0.8 kg initial BW) were allocated to three treatments, each for 30 d: (i) control group (CG), basal diet containing Zn-free premix + 100 mg Zn/kg from ZnSO4; (ii) high Zn (HZN), basal diet containing Zn-free premix + 2,250 mg Zn/kg from ZnO; (iii) coated nano ZnO (CNZO), basal diet containing Zn-free premix + 100 mg Zn/kg from coated NZO. In Exp.2, 21 crossbred growing pigs (17.04 +/- 0.01 kg initial BW) were allocated to three treatments, each for 28 d: (i) HZN, basal diet containing Zn-free premix + 2,250 mg Zn/kg from ZnO; (ii) low concentration of nano ZnO (LNZO), basal diet containing Zn-free premix + 100 mg Zn/ kg from 5% coated NZO material; (iii) high concentration of nano ZnO (HNZO), basal diet containing Zn-free premix + 100 mg Zn/kg from 10% coated NZO material. In Exp. 1, compared with the CG diet, CNZO significantly reduced the diarrhea rate (P < 0.05) and increased the activities of glutathione peroxidase and superoxide dismutase (P < 0.05). Compared with HZN, CNZO decreased the activities of serum alanine aminotransferase, and alkaline phosphatase, as well as the fecal zinc concentration (P < 0.05). In Exp. 2, pigs fed LNZO or HNZO had an increased final BW, average daily weigh and diarrhea rate, and a decreased level of Zn in the plasma, liver, and feces on day 14 compared with the HZN group (P < 0.05). The villous height and villous height/crypt depth ratio of duodenum were higher (P < 0.05) in the HZN group than the HNZO group, whereas the higher villous height of jejunum was observed in the LNZO group compared with that in the HNZO group (P < 0.05). We found that CNZO (100 mg/kg Zn) could improve the antioxidant capacity and reduce fecal Zn emission. However, the diarrhea rate was not effectively suppressed when compared with the HNZO supplementation. Furthermore, coated NZO material of 5% concentration is more effective in improving the morphology of intestinal villus.