Cell-to-Cell Transcription Variability as Measured by Single-Molecule RNA FISH to Detect Epigenetic State Switching.
Cell-to-Cell Transcription Variability as Measured by Single-Molecule RNA FISH to Detect Epigenetic State Switching.
复制标题
通过单分子 RNA FISH 测量细胞间转录变异，以检测表观遗传状态转换。
DOI：
10.1007/978-1-4939-7774-1_21
复制
发表时间：
2018
影响因子：
--
通讯作者：
P. J. Verschure
中科院分区：
文献类型：
--
作者：
W. Beckman;Ilona M. Vuist;Hermannus Kempe;P. J. Verschure
Single-molecule RNA fluorescent in situ hybridization (smRNA FISH) allows for the visualization, localization, and quantification of RNA transcripts within individual cells and tissues using custom-designed fluorescently labeled oligonucleotide probes. Here we describe a protocol for the preparation, imaging, and analysis of a smRNA FISH experiment that can be applied to any RNA of choice. We also provide insights as to how this powerful tool can be used to study epigenetic regulation, for example, following the epigenetic editing of genes.
