Fission Yeast Germinal Center (GC) Kinase Ppk11 Interacts with Pmo25 and Plays an Auxiliary Role in Concert with the Morphogenesis Orb6 Network (MOR) in Cell Morphogenesis
Fission Yeast Germinal Center (GC) Kinase Ppk11 Interacts with Pmo25 and Plays an Auxiliary Role in Concert with the Morphogenesis Orb6 Network (MOR) in Cell Morphogenesis
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DOI：
10.1074/jbc.m110.176867
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发表时间：
2010-11-05
影响因子：
4.8
通讯作者：
Hirata, Dai
中科院分区：
文献类型：
--
作者：
Goshima, Tetsuya;Kume, Kazunori;Hirata, Dai
细胞形态和细胞周期如何协调调节是细胞生物学的一个基本课题。在裂殖酵母中，2种生发中心激酶（GCK），Sid 1和Nak 1，分别作为分隔起始网络（SIN）和形态发生Orb 6网络（莫尔）的组成部分在分隔/胞质分裂和细胞分离/细胞极性控制中发挥重要作用。在这里，我们表明，第三GCK，PPK 11，也需要有效的细胞分离，特别是在高温下。虽然Ppk 11不是细胞分裂所必需的，但这种激酶在细胞形态发生中与莫尔一起起辅助作用。Ppk 11与莫尔组分Pmo 25物理相互作用并定位于隔膜，由此Ppk 11对于Pmo 25靶向/积聚到隔膜至关重要。Ppk 11保守的C-末端WDF基序对于Pmo 25的隔膜积累和有效的细胞分离都是必不可少的。相反，它的激酶活性仅用于细胞分离。因此，Ppk 11与Pmo 25的相互作用和Ppk 11激酶活性对于有效的细胞分离都是至关重要的。
How cell morphology and the cell cycle are coordinately regulated is a fundamental subject in cell biology. In fission yeast, 2 germinal center kinases (GCKs), Sid1 and Nak1, play an essential role in septation/cytokinesis and cell separation/cell polarity control, respectively, as components of the septation initiation network (SIN) and the morphogenesis Orb6 network (MOR). Here we show that a third GCK, Ppk11, is also required for efficient cell separation particularly, at a high temperature. Although Ppk11 is not essential for cell division, this kinase plays an auxiliary role in concert with MOR in cell morphogenesis. Ppk11 physically interacts with the MOR component Pmo25 and is localized to the septum, by which Ppk11 is crucial for Pmo25 targeting/accumulation to the septum. The conserved C-terminal WDF motif of Ppk11 is essential for both septum accumulation of Pmo25 and efficient cell separation. In contrast its kinase activity is required only for cell separation. Thus, both interaction of Ppk11 with Pmo25 and Ppk11 kinase activity are critical for efficient cell separation.