DEVELOPMENT OF THE CALCIUM-BINDING PROTEINS PARVALBUMIN AND CALBINDIN IN MONKEY STRIATE CORTEX
DEVELOPMENT OF THE CALCIUM-BINDING PROTEINS PARVALBUMIN AND CALBINDIN IN MONKEY STRIATE CORTEX
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DOI：
10.1002/cne.903070409
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发表时间：
1991-05-22
影响因子：
2.5
通讯作者：
CELIO, MR
中科院分区：
文献类型：
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作者：
HENDRICKSON, AE;VANBREDERODE, JFM;CELIO, MR
研究了猕猴纹状皮质中从胎儿 (F) 60 天到产后 (P) 5 年以上的钙结合蛋白小白蛋白 (PV) 和钙结合蛋白-D28K (Cal) 的免疫反应性发展。我们将 PV 和 Cal 染色模式的变化与有据可查的灵长类纹状皮层神经元生成和成熟、突触发生和丘脑皮质轴突相互作用的发育序列相关联，试图推断出这些蛋白质的功能作用。我们的主要发现是，Cal 和 PV 具有截然相反的发育模式，除了第 1 层。在 F60 天，两者仅存在于第 1 层神经元中，并且标记的细胞体和过程的数量增加到F125天。第 1 层中几乎所有 Cal+ 和 PV+ 细胞在第 12 周时消失。 Cal 在 F113 天时出现在锥体和星状神经元中，特别是第 4-6 层。第 2-6 层细胞的数量和染色密度在出生前增加，然后在 P9-12 周时均下降。颗粒上层显示 Cal 标记从 P20-36 周开始第二次增加，然后缓慢下降至 P1-2 岁时达到的成人模式。在产前和产后早期，4A、4C-α 层和深 4C-β 层的细胞体含有大量 Cal+，但上层 4C-β 仍未标记。直到 F155-162 天，才在 2-6 层中看到 PV。大星状细胞和一些锥体细胞首先出现在第 5/6 层和 4C-α 层中，但到 P6 周时，PV+ 细胞星状神经元在除 4C-β 层以外的所有层中均发现。 P3周时4C-β层含有少量PV+细胞体，并且光神经丛4C-β超过4C-α。到 P20 周时，纹状皮层的细胞数量和神经丛密度已达到成人模式。这些发育模式表明，Cal 细胞体染色的最高密度与突触发生或视觉驱动的双眼相互作用的出生后关键期无关。相反，当外侧膝状体轴突到达皮质时，Cal 就会出现，在丘脑皮质相互作用的整个过程中持续存在，并在皮质可塑性下降期间消失。 PV 的出现与出生时复杂的视觉驱动活动的开始高度相关，而 PV+ 细胞体的数量和 PV+ 神经丛的密度都达到成人水平，与丘脑皮质连接的完成一致。
The development of immunoreactivity for the calcium-binding proteins parvalbumin (PV) and calbindin-D28K (Cal) was studied in Macaca nemestrina striate cortex from fetal (F) 60 days to postnatal (P) 5+ years. We correlated changes in PV and Cal staining patterns with the well-documented developmental sequence for primate striate cortex neuron generation and maturation, synaptogenesis, and thalamocortical axon interactions in an attempt to deduce a functional role for these proteins.Our major finding is that Cal and PV have diametrically opposed developmental patterns except in layer 1. At F60 days both are present only in neurons of layer 1 and the number of labeled cell bodies and processes increases up to F125 days. Almost all Cal+ and PV+ cells in layer 1 disappear by P12 weeks. Cal is present by F113 days in pyramidal and stellate neurons, particularly layers 4-6. The number and staining density of cells in layers 2-6 increases up to birth and then both decline by P9-12 weeks. Supragranular layers show a second increase in Cal labeling from P20-36 weeks, and then there is a slow decline to the adult pattern which is reached by P1-2 years. Cell bodies in layers 4A, 4C-alpha, and deep 4C-beta are heavily Cal+ during pre-and early post-natal periods, but upper 4C-beta remains unlabeled.PV is not seen until F155-162 days in layers 2-6. Large stellate and a few pyramidal cells appear first in layers 5/6 and 4C-alpha, but PV+ cell stellate neurons are found in all layers except 4C-beta by P6 weeks. Layer 4C-beta contains a few PV+ cell bodies at P3 weeks, and light neuropile 4C-beta exceeds that of 4C-alpha. Striate cortex has an adult pattern of cell number and neuropile density by P20 weeks.These developmental patterns suggest that the highest density of Cal cell body staining does not correlate with synaptogenesis, or the postnatal critical period of visually driven, binocular interactions. Rather Cal appears when lateral geniculate axons arrive in cortex, persists over the entire span of thalamocortical interactions, and disappears during the decline of cortical plasticity. The appearance of PV is highly correlated with the onset of complex visually driven activity at birth, while both the number of PV+ cell bodies and the density of PV+ neuropile reach adult levels coincident with the completion of thalamocortical connections.