CD300 antigen like family member G: A novel Ig receptor like protein exclusively expressed on capillary endothelium
CD300 antigen like family member G: A novel Ig receptor like protein exclusively expressed on capillary endothelium
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DOI：
10.1016/j.bbrc.2006.07.047
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发表时间：
2006-09-15
影响因子：
3.1
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
--
作者：
Takatsu, Hiroyuki;Hase, Koji;Ohno, Hiroshi
我们报告了 CD300LG（CD300 抗原样家族成员）的特征。小鼠和人类的基因组结构相似，并且两个物种中至少存在四种亚型。 CD300LG 的免疫球蛋白 (Ig) V 样结构域的氨基酸序列与聚合 Ig 受体 (pIgR) 和 Fc α/mu R 的氨基酸序列具有约 35% 的同一性。有趣的是，小鼠 CD300LG 蛋白在毛细血管内皮上独特表达。免疫电镜显示小鼠 CD300LG 定位于顶端和基底外侧质膜，以及毛细血管内皮细胞内的囊泡结构。使用极化 MDCK 上皮细胞的转胞吞测定表明 CD300LG 可以双向转胞吞。此外，HeLa细胞上外源表达的CD30OLG可以摄取IgA2和IgM，但不能摄取IgG。这些结果表明 CD30OLG 可能在穿过毛细血管内皮的分子运输中发挥重要作用。 (c) 2006 Elsevier Inc. 保留所有权利。
We report the characteristics of CD300LG, a member of the CD300 antigen like family. Its genomic structure is similar in both mouse and human, and at least four isoforms exist in both species. The amino acid sequence of the immunoglobulin (Ig) V like domain of CD300LG showed approximately 35% identity to those of the polymeric Ig receptor (pIgR) and Fc alpha/mu R. Interestingly, mouse CD300LG proteins were uniquely expressed on capillary endothelium. Immunoelectron microscopy revealed that mouse CD300LG is localized on both apical and basolateral plasma membranes, as well as on intracellular vesicular structures, in the capillary endothelium. Transcytosis assays using polarized MDCK epithelial cells showed that CD300LG could be transcytosed bidirectionally. Furthermore, CD30OLG exogenously expressed on HeLa cells could take up IgA2 and IgM, but not IgG. These results suggest that CD30OLG might play an important role in molecular traffic across the capillary endothelium. (c) 2006 Elsevier Inc. All rights reserved.