Toxic response of nickel nanoparticles in human lung epithelial A549 cells
Toxic response of nickel nanoparticles in human lung epithelial A549 cells
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DOI：
10.1016/j.tiv.2011.02.015
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发表时间：
2011-06-01
影响因子：
3.2
通讯作者：
Ahamed, Maqusood
中科院分区：
文献类型：
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作者：
Ahamed, Maqusood
纳米镍在催化剂、传感器和电子等现代工业中得到越来越广泛的应用。由于广泛的工业应用，吸入是暴露于Ni NPs的主要来源。然而，显示Ni NPs对肺系统影响的数据仍然很少。本研究旨在检测Ni NPs在0、1、2、5、10和25 μ g/ml浓度下对人肺上皮A549细胞的毒性作用，并以线粒体功能（mu）、乳酸脱氢酶（LDH）、还原性谷胱甘肽（GSH）、活性氧（ROS）、膜脂过氧化（LPO）和caspase-3活性作为毒性终点。结果表明，Ni NPs降低线粒体功能，并诱导LDH渗漏呈剂量依赖性和时间依赖性。Ni NPs还通过GSH的消耗和ROS和LPO的诱导，以剂量和时间依赖的方式诱导氧化应激。细胞凋亡标志物caspase-3酶活性随时间和Ni NPs剂量的增加而升高。结果表明，Ni NPs对人肺上皮A549细胞具有明显的毒性，其毒性可能是通过氧化应激介导的。这项研究保证在工业应用之前对Ni NPs进行更仔细的评估。(C) 2011 Elsevier Ltd.版权所有。
Nickel nanoparticle (Ni NP) is increasingly used in modern industries such as catalysts, sensors and electronic applications. Due to wide-spread industrial applications the inhalation is the primary source of exposure to Ni NPs. However, data demonstrating the effect of Ni NPs on the pulmonary system remain scarce. The present study was designed to examine the toxic effect of human lung epithelial A549 cells treated with well characterized Ni NPs at the concentrations of 0, 1, 2, 5, 10 and 25 mu g/ml for 24 and 48 h. Mitochondrial function (MU assay), membrane leakage of lactate dehydrogenase (LDH assay), reduced glutathione (GSH), reactive oxygen species (ROS), membrane lipid peroxidation (LPO) and caspase-3 activity were assessed as toxicity end points. Results showed that Ni NPs reduced mitochondrial function and induced the leakage of LDH in dose and time-dependent manner. Ni NPs were also found to induce oxidative stress in dose and time-dependent manner indicated by depletion of GSH and induction of ROS and LPO. Further, activity of caspase-3 enzyme, marker of apoptosis was significantly higher in treated cells with time and Ni NPs dosage. The results exhibited significant toxicity of Ni NPs in human lung epithelial A549 cells which is likely to be mediated through oxidative stress. This study warrants more careful assessment of Ni NPs before their industrial applications. (C) 2011 Elsevier Ltd. All rights reserved.