Antitumor and Adjuvant Activity of λ-carrageenan by Stimulating Immune Response in Cancer Immunotherapy.
Antitumor and Adjuvant Activity of λ-carrageenan by Stimulating Immune Response in Cancer Immunotherapy.
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DOI：
10.1038/srep11062
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发表时间：
2015-06-22
影响因子：
4.6
通讯作者：
Wei YQ
中科院分区：
文献类型：
--
作者：
Luo M;Shao B;Nie W;Wei XW;Li YL;Wang BL;He ZY;Liang X;Ye TH;Wei YQ
λ-卡拉胶是一种海藻多糖，在基础研究中被广泛用作促炎剂，但λ-卡拉胶的免疫调节活性如何影响肿瘤微环境尚不清楚。本研究发现，瘤内注射λ-卡拉胶可抑制B16-F10和4 T1荷瘤小鼠的肿瘤生长，并通过增加肿瘤浸润的M1巨噬细胞、树突状细胞和脾脏中活化的CD 4 + CD 8 + T淋巴细胞的数量来增强肿瘤免疫应答。此外，λ-卡拉胶还能增强脾脏IL 17 A的分泌，显著提高肿瘤组织TNF-α的水平，其中大部分由浸润的巨噬细胞分泌。此外，λ-卡拉胶在用于癌症治疗的基于OVA的预防性和治疗性疫苗中表现出有效的佐剂作用，这显著增强了抗OVA抗体的产生。毒性分析表明λ-卡拉胶具有良好的安全性。因此，λ-卡拉胶可能既作为一种有效的抗肿瘤剂，又作为一种有效的癌症免疫治疗佐剂。
λ-Carrageenan is a seaweed polysaccharide which has been generally used as proinflammatory agent in the basic research, however, how the immunomodulating activity of λ-carrageenan affects tumor microenvironment remains unknown. In this study, we found that intratumoral injection of λ-carrageenan could inhibit tumor growth in B16-F10 and 4T1 bearing mice and enhance tumor immune response by increasing the number of tumor-infiltrating M1 macrophages, DCs and more activated CD4+CD8+ T lymphocytes in spleen. In addition, λ-carrageenan could enhance the secretion of IL17A in spleen and significantly increase the level of TNF-α in tumor, most of which was secreted by infiltrating macrophages. Moreover, λ-carrageenan exhibited an efficient adjuvant effect in OVA-based preventative and therapeutic vaccine for cancer treatment, which significantly enhanced the production of anti-OVA antibody. The toxicity analysis suggested that λ-carrageenan was with a good safety profile. Thus, λ-carrageenan might be used both as a potent antitumor agent and an efficient adjuvant in cancer immunotherapy.
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通过淋巴结消除来清除稳态细胞因子下沉，增强了采用转移的肿瘤特异性CD8+ T细胞的功效。
影响因子：
15.3
作者：
Gattinoni, Luca;Finkelstein, Steven E;Klebanoff, Christopher A;Antony, Paul A;Palmer, Douglas C;Spiess, Paul J;Hwang, Leroy N;Yu, Zhiya;Wrzesinski, Claudia;Heimann, David M;Surh, Charles D;Rosenberg, Steven A;Restifo, Nicholas P
通讯作者：
Restifo, Nicholas P
影响因子：
20.3
作者：
Kryczek, Ilona;Banerjee, Mousumi;Zou, Weiping
通讯作者：
Zou, Weiping
影响因子：
15.9
作者：
Narayanan, Priyadharshini;Lapteva, Natalia;Spencer, David M.
通讯作者：
Spencer, David M.
影响因子：
11.2
作者：
Jung, In Duk;Jeong, Soo Kyung;Park, Yeong-Min
通讯作者：
Park, Yeong-Min
影响因子：
3.1
作者：
Malley, R;Srivastava, A;Anderson, PW
通讯作者：
Anderson, PW