Application of fluid mechanical force to embryonic sources of hemogenic endothelium and hematopoietic stem cells.
Application of fluid mechanical force to embryonic sources of hemogenic endothelium and hematopoietic stem cells.
复制标题
流体机械力在造血内皮和造血干细胞胚胎来源中的应用。
DOI：
10.1007/7651_2014_95
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Wenzel,PamelaL
中科院分区：
文献类型：
--
作者：
Li,Nan;Diaz,MiguelF;Wenzel,PamelaL
在胚胎发育过程中，血流引起的血流动力学支持血管重塑、管腔内皮的动脉化和造血干细胞（HSC）的出现。之前，我们报道了流体剪切应力在刺激主动脉-性腺-中肾（AGM）中的一氧化氮（NO）信号传导中起着关键作用，并且对于确定的造血至关重要。我们采用了由锥板组件改造而来的动态流动系统，以精确调节 AGM 细胞在体外暴露于特定模式的层流剪切应力。在这里，我们提出了一种微流体平台的设计，任何研究小组都可以使用，该平台需要少量的细胞，并允许旁分泌信号因子的再循环，同时对非贴壁造血祖细胞和干细胞的损害最小。我们详细介绍了使用市售组件组装微流体平台，并为使用新兴标准来测量胚胎 HSC 潜力（静脉新生儿移植）提供具体指导。
During embryonic development, hemodynamic forces caused by blood flow support vascular remodeling, arterialization of luminal endothelium, and hematopoietic stem cell (HSC) emergence. Previously, we reported that fluid shear stress plays a key role in stimulating nitric oxide (NO) signaling in the aorta-gonad-mesonephros (AGM) and is essential for definitive hematopoiesis. We employed a Dynamic Flow System modified from a cone-and-plate assembly to precisely regulate in vitro exposure of AGM cells to a defined pattern of laminar shear stress. Here, we present the design of a microfluidic platform accessible to any research group that requires small cell numbers and allows for recirculation of paracrine signaling factors with minimal damage to nonadherent hematopoietic progenitors and stem cells. We detail the assembly of the microfluidic platform using commercially available components and provide specific guidance in the use of an emerging standard in the measurement of embryonic HSC potential, intravenous neonatal transplantation.