Incorporation of fatty acids and amino acids by cultured Pneumocystis carinii.
Incorporation of fatty acids and amino acids by cultured Pneumocystis carinii.
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培养的卡氏肺囊虫掺入脂肪酸和氨基酸。
DOI：
10.1111/j.1550-7408.1994.tb01525.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Queener,SF
中科院分区：
文献类型：
--
作者：
Paulsrud,JR;Queener,SF
CulturedP. cariniirapidly took up a variety of fatty acids. The relative rates of uptake for four fatty acids were 18:1 >> 16:0 ≅ 18:0 ≅ 18:2. Fatty acids were primarily incorporated into phospholipids and the uptake process was specifically inhibited by 2.2 and 22 μM primaquine, a concentration having no effect on host cells. Amino acids were also taken up by culturedP. cariniiin a primaquine sensitive process. Radiolabeled leucine was incorporated into the major surface glycoprotein ofP. carinii.The formation of radioactiveP. carinii‐specific proteins indicated that the cultured organisms carried out transcription and translation and that the incorporation of amino acids was dependent uponP. cariniirather than rare HEL human embryonic lung cells. The spinner flask culture system provides convenient access toP. cariniifor metabolic studies in defined medium for a period of 5–14 days after inoculation.
