δ-Tocopherol promotes thermogenic gene expression via PGC-1α upregulation in 3T3-L1 cells
δ-Tocopherol promotes thermogenic gene expression via PGC-1α upregulation in 3T3-L1 cells
复制标题
DOI：
10.1016/j.bbrc.2018.10.021
复制
发表时间：
2018-11-17
影响因子：
3.1
通讯作者：
Kiyose, Chikako
中科院分区：
文献类型：
--
作者：
Tanaka-Yachi, Rieko;Shirasaki, Masato;Kiyose, Chikako
生热脂肪细胞(棕色和米色)的激活被认为是减肥和治疗代谢性疾病的一个有吸引力的靶点。过氧化物酶体增殖物激活受体γ辅活化子1α(PGC1-α)是生热脂肪细胞产热基因表达的主要调节因子。我们先前报道，α-生育酚上调PGC-1α基因的表达，促进哺乳动物脂肪细胞的生热脂肪细胞分化。在这项研究中，我们研究了维生素E类似物(α、γ和β生育酚)对3T3-L1细胞PGC-Lα和解偶联蛋白1(UCP1)基因表达的影响。随着β-生育酚的加入，PGC-1α和UCP1的表达显著增加。在增量生育酚处理的细胞中，PGC-1α的核转位增加，p38丝裂原活化蛋白激酶(MAPK)的表达和磷酸化也增加。我们的结果表明，S生育酚激活p38MAPK参与了PGC-1a的激活和UCP1的诱导。(C)2018 Elsevier Inc.保留所有权利。
Activation of thermogenic adipocytes (brown and beige) has been considered an attractive target for weight loss and treatment of metabolic disease. Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC1-alpha) is a master regulator of thermogenic gene expression in thermogenic adipocytes. We previously reported that alpha-tocopherol upregulated PGC-1 alpha gene expression and promoted thermogenic adipocyte differentiation in mammalian adipocytes. In this study, we investigated the effects of the vitamin E analogs (alpha-, gamma- and delta-tocopherol) on PGC-l alpha and uncoupling protein 1 (UCP1) gene expression in 3T3-L1 cells. The expression of PGC-1 alpha and UCP1 increased significantly with the addition of delta-tocopherol. In delta-tocopherol-treated cells, nuclear translocation of PGC-1 alpha increased, as did p38 mitogen-activated protein kinase (MAPK) expression and phosphorylation. Our results suggest that p38 MAPK activation by S-tocopherol contributes to PGC-la activation and UCP1 induction. (C) 2018 Elsevier Inc. All rights reserved.