Impacts of biochar on the environmental risk of antibiotic resistance genes and mobile genetic elements during anaerobic digestion of cattle farm wastewater.
Impacts of biochar on the environmental risk of antibiotic resistance genes and mobile genetic elements during anaerobic digestion of cattle farm wastewater.
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DOI：
10.1016/j.biortech.2018.02.052
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发表时间：
2018-05
影响因子：
11.4
通讯作者：
Wei Sun;Jie Gu;Xiaojuan Wang;Xun Qian;Xiaxia Tuo
中科院分区：
文献类型：
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作者：
Wei Sun;Jie Gu;Xiaojuan Wang;Xun Qian;Xiaxia Tuo
生物炭对厌氧消化过程中的氮保存具有积极作用，但其对抗生素抗性基因（ARG）的影响尚不清楚。因此，研究了生物炭（0、5、20和50g/L）对牛粪废水厌氧消化过程中ARGs环境风险的影响。结果表明，5g/L生物炭降低了消化产物中5/13个ARG的相对丰度（RA），而20g/L生物炭则显着降低了消化产物中ARG的总RA，其中ISCR1的RA比对照低0.89个log。生物炭主要通过影响厚壁菌门和变形菌门的RA来影响ARGs的分布，且20g/L生物炭的影响大于5g/L。移动遗传元件也影响 ARG 谱，尤其是 I2 和 ISCR1。在牛场废水厌氧消化系统中添加20g/L生物炭可以降低ARGs的环境风险。
Biochar has positive effects on nitrogen conservation during anaerobic digestion, but its impacts on antibiotic resistance genes (ARGs) are unclear. Therefore, the effect of biochar (0, 5, 20, and 50 g/L) on the environmental risk of ARGs during cattle manure wastewater anaerobic digestion were investigated. The results showed that 5 g/L biochar reduced the relative abundances (RAs) of 5/13 ARGs while 20 g/L biochar significantly reduced the total RAs of ARGs in the digestion products, where the RA of ISCR1was 0.89 log lower than the control. Biochar mainly affected the distribution of ARGs by influencing the RAs of Firmicutes and Proteobacteria, and the influence of 20 g/L biochar was greater than that of 5 g/L. Mobile genetic elements also influenced the ARG profiles, especiallyintI2and ISCR1. The addition of 20 g/L biochar to cattle farm wastewater anaerobic digestion systems could reduce the environmental risk of ARGs.