Optical flow of vesicles: computer vision approach for endocytosis of Nano Particles in a living cell.
Optical flow of vesicles: computer vision approach for endocytosis of Nano Particles in a living cell.
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囊泡光流：活细胞中纳米颗粒内吞作用的计算机视觉方法。
DOI：
10.1117/12.2543455
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
and Masatoshi Ishikawa.
中科院分区：
文献类型：
--
作者：
Seohyun Lee*;Hyuno Kim;Hideo Higuchi;and Masatoshi Ishikawa.
活细胞中囊泡的运动包括了解细胞内运输细节的重要信息。尽管囊泡追踪方法使我们能够从物理角度理解单个纳米颗粒的精确运动，但仅通过分析几个有代表性的囊泡运动仍然无法清楚地解释整个细胞水平的运输。在本研究中，作为深入了解细胞水平囊泡运输的初步尝试，我们采用计算机视觉技术来分析整个细胞内囊泡运输。详细地说，我们提出了一种算法，根据相对于几何细胞中心的收敛和发散来估计和可视化整个内吞囊泡的流动。在此算法中，使用 Lucas-Kanade 方法计算活细胞中荧光纳米粒子的光流。然后，根据四象限反正切，计算并绘制关于细胞几何中心的囊泡运动方向，以可视化会聚或发散运动。通过这种建议的方法，我们有望深入了解细胞水平的囊泡运输，这可以帮助设计和定量评估各种生物医学应用，包括药物输送。
The movement of vesicle in a living cell includes essential information for understanding the details of the intracellular transport. Although the vesicle tracking method has allowed us to understand precise movement of a single nanoparticle from the physical point of view, the whole cell-level transport has still not been clearly explained with the analysis of only a few representative vesicle movements. In this study, as an initial attempt to gain insight into cell-level vesicle transport, we adopted a computer vision technique to analyze the overall intracellular vesicle transport. In detail, we propose an algorithm to estimate and visualize the ow of the entire endocytic vesicles in terms of convergence and divergence with respect to the geometric cell center. In this algorithm, optical ow of the fluorescent nanoparticles in a living cell is computed using Lucas-Kanade method. Then, the direction of vesicle movement regarding the geometric center of the cell is calculated and mapped to visualize either converging or diverging movement, based on four-quadrant inverse tangent. With this suggested method, it is expected that we can gain insight into cell-level vesicle transport, which can help design and quantitatively evaluate various biomedical applications, including drug delivery.