Increased levels of circulating soluble CD14 but not CD83 in infants are associated with early intestinal colonization with Staphylococcus aureus
Increased levels of circulating soluble CD14 but not CD83 in infants are associated with early intestinal colonization with Staphylococcus aureus
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DOI：
10.1111/j.1365-2222.2006.02625.x
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发表时间：
2007-01-01
影响因子：
6.1
通讯作者：
Rudin, A.
中科院分区：
文献类型：
--
作者：
Lundell, A-C.;Adlerberth, I.;Rudin, A.
可溶形式的单核细胞标记物 CD14 和成熟树突状细胞标记物 CD83 是具有免疫调节功能的血浆蛋白。它们产生的生理刺激尚不清楚，它们在过敏发展中的可能作用也不清楚。我们测量了 64 名瑞典儿童的血浆可溶性 CD14 (sCD14) 和可溶性 CD83 (sCD83) 水平，并与前瞻性出生队列中的肠道细菌定植模式相关。通过酶联免疫吸附测定对出生时以及 4、18 和 36 月龄时获得的血浆中可溶性 CD14 和 sCD83 水平进行定量。对生命前 8 周定期采集的粪便样本中的所有主要需氧和厌氧细菌进行了定量。在 18 个月大时评估临床过敏和 IgE 水平。血浆中的可溶性 CD14 在生命的前 18 个月内增加，而 sCD83 在 4 个月大时达到峰值。与未定植的儿童相比，围产期定植有金黄色葡萄球菌的儿童在 4 个月大时血浆中 sCD14 的水平显着升高。 sCD14的水平与大肠杆菌、其他肠杆菌、肠球菌、梭菌、拟杆菌、双歧杆菌或乳酸杆菌的定植无关。此外，18 个月大时患有食物过敏的儿童的 sCD14 水平往往低于健康儿童。 sCD83 的血浆水平与细菌定植模式或过敏发生无关。围产期金黄色葡萄球菌定植可能会触发血浆中 sCD14 的出现，这可能会影响婴儿免疫系统的发育和过敏发生的风险。
Soluble forms of the monocyte marker CD14 and the mature dendritic cell marker CD83 are plasma proteins with immunoregulatory functions. The physiological stimulus for their production is unclear and their possible role in allergy development is unknown.We measured the plasma levels of soluble CD14 (sCD14) and soluble CD83 (sCD83) in 64 Swedish children in relation to intestinal bacterial colonization pattern in a prospective birth cohort. Soluble CD14 and sCD83 levels were quantified by enzyme linked immunosorbent assay in plasma obtained at birth and at 4, 18 and 36 months of age. All major aerobic and anaerobic bacteria were quantified in faecal samples obtained regularly over the first 8 weeks of life. Clinical allergy and IgE levels were evaluated at 18 months of age.Soluble CD14 in plasma increased during the first 18 months of life while sCD83 peaked at 4 months of age. Children who were perinatally colonized with Staphylococcus aureus had significantly higher levels of sCD14 in plasma at 4 months of age relative to non-colonized children. The levels of sCD14 were unrelated to colonization with Escherichia coli, other enterobacteria, enterococci, clostridia, Bacteroides, bifidobacteria or lactobacilli. Further, children with food allergy by 18 months tended to have lower levels of sCD14 than healthy children. Plasma levels of sCD83 were not related to either bacterial colonization pattern or allergy development.Perinatal colonization with S. aureus may trigger the occurrence of sCD14 in plasma, which may influence development of the infantile immune system and risk of allergy development.