A CRITICAL EVALUATION OF THE NITROGEN ASSIMILATION TESTS COMMONLY USED IN THE CLASSIFICATION OF YEASTS
A CRITICAL EVALUATION OF THE NITROGEN ASSIMILATION TESTS COMMONLY USED IN THE CLASSIFICATION OF YEASTS
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DOI：
10.1128/jb.52.3.293-301.1946
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发表时间：
1946-01-01
影响因子：
3.2
通讯作者：
WICKERHAM, LJ
中科院分区：
文献类型：
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作者：
WICKERHAM, LJ
酵母同化（NH4）2SO4、尿素、天冬酰胺或蛋白胨的能力。已被广泛用作分类中的诊断测试。过去，培养基仅含有磷酸二氢钾、MgSO4、葡萄糖和一种N源。在此培养基中添加8种纯B族维生素、微量元素、NaCl和CaCl 2，所有的种都能生长。发现以前被许多作者认为不能同化（NH4）2SO4、尿素和天冬酰胺的植物能够同化。当省略维生素时，发现这些物种中只有一种能够继续生长，从而证明在先前使用的培养基中缺乏维生素阻止了某些物种的生长，而不是不能利用培养基中的特定N源。
The ability of yeasts to assimilate (NH4)2SO4, urea, asparagine, or peptone. from a synthetic medium has been widely used as diagnostic tests in classification. In the past, the medium contained only potassium dihydrogen phosphate, MgSO4, glucose, and one of the N sources. By addition to this medium of 8 pure B vitamins, trace elements, NaCl and CaCl2, all spp. that were studied which previously had been designated by various authors as not able to assimilate (NH4)2SO4, urea, and asparagine were found to do so. When the vitamins were omitted, only one of these species was found capable of continued growth, thus demonstrating that it was the lack of vitamins in the previously used medium which prevented growth from occurring with some spp., and not an inability to use the particular N source in the medium.