Differential behavior of curved DNA upon untwisting
Differential behavior of curved DNA upon untwisting
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DOI：
10.1073/pnas.94.2.403
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发表时间：
1997-01-21
影响因子：
11.1
通讯作者：
Chartrand, P
中科院分区：
文献类型：
--
作者：
Brukner, I;Belmaaza, A;Chartrand, P
我们合成了不同旋向、扭曲度和曲率半径的DNA片段，并分析了去扭曲对它们的影响。结果表明，弯曲DNA解扭后的动力学行为强烈地取决于初始序列依赖的DNA轨迹。特别是，具有相同半径但具有相反旋向的超螺旋扭曲的DNA可以对溴化乙锭解旋显示出非常不同的构象响应。在用溴化乙锭处理后，右手超螺旋降低它们的扭曲并增加超螺旋的平面性，而左手超螺旋增加扭曲并降低它们的平面性程度。
We have synthesized DNA segments with different handedness, twisting and radii of curvature, and have analyzed the effect of untwisting on them. The results indicate that the dynamic behavior of curved DNA upon untwisting is strongly determined by the initial sequence-dependent DNA trajectory. In particular, DNA with the same radii but with opposite handedness of superhelix twisting can show very different conformational responses to ethidium bromide untwisting. Upon treatment with ethidium bromide, right-handed superhelixes decrease their twist and increase the planarity of the superhelix, while left-handed superhelixes increase twisting and decrease their degree of planarity.