Metabolism and Translocation of Gibberellins in Seedlings of Pharbitis nil. (I) Effect of Photoperiod on Stem Elongation and Endogenous Gibberellins in Cotyledons and Their Phloem Exudates
Metabolism and Translocation of Gibberellins in Seedlings of Pharbitis nil. (I) Effect of Photoperiod on Stem Elongation and Endogenous Gibberellins in Cotyledons and Their Phloem Exudates
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牵牛幼苗中赤霉素的代谢和易位。
DOI：
10.1093/oxfordjournals.pcp.a078753
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发表时间：
1995
影响因子：
4.9
通讯作者：
N. Murofushi
中科院分区：
文献类型：
--
作者：
Young;I. Yamaguchi;Kiyotoshi Takeno;Yoshihito Suzuki;N. Murofushi
牵牛树幼苗韧皮部分泌物和子叶中的赤霉素A1、(GA1)、GA19和GA20。通过高效液相色谱 (HPLC) 和放射免疫分析 (RIA) 相结合，对在不同光周期条件下生长的紫罗兰进行定性和半定量分析。在 16 小时光照/8 小时黑暗 6 天，然后 8 小时光照/16 小时黑暗 3 天的黑暗处理 (DT) 条件下生长的植物的子叶中 GA19 和 GA20 的水平高于在连续光照 (CL) 下生长 9 天的植物。韧皮部分泌物中的 GA 也观察到了这种关系，尽管其水平远低于子叶中的水平。当将 GA 施用于子叶时，GA20 比 GA1 或 GA19 更能促进上胚轴的伸长，特别是在 CL 处理下。当将这些 GA 应用于用调环酸（2-酮戊二酸依赖性双加氧酶抑制剂）预处理的上胚轴时，GA1 和 GA20 对上胚轴伸长的相对影响发生逆转。
Gibberellin A1, (GA1), GA19, and GA20in phloem exudates and cotyledons of seedlings ofPharbitis nilcv. Violet, grown under different photoperiodic conditions, were qualitatively and semi-quantitatively analyzed by a combination of high performance-liquid chromatography (HPLC) and radioimmunoassays (RIA). The levels of GA19and GA20were higher in cotyledons from plants grown under dark treatment (DT) conditons of 16 h-light/8 h-dark for 6 days followed by 8 h-light/16 h-dark for 3 days than in those grown under continuous light (CL) for 9 days. This relationship was also observed for the GAs in phloem exudates, although the levels were much lower than in the cotyledons. When GAs were applied to the cotyledons, elongation of the epicotyl was promoted more by GA20than by GA1or GA19, especially under the CL treatment. The relative effect of GA1and GA20on the epicotyl elongation was reversed when these GAs were applied to epicotyls pre-treated with prohexadione, an inhibitor of 2-oxoglutarate-dependent dioxygenases.