Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana
Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana
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DOI：
10.1111/j.1440-169x.2009.01135.x
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发表时间：
2009-12-01
影响因子：
2.5
通讯作者：
Kamei, Yasuhiro
中科院分区：
文献类型：
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作者：
Deguchi, Tomonori;Itoh, Mariko;Kamei, Yasuhiro
热激启动子是精确调控外源基因在生物体内诱导的有力工具。除了基因表达的时间控制，基因功能的分析也可能需要空间限制。最近，我们报道了一种新的方法，在体内，单细胞基因诱导使用红外激光诱发的基因操作（IR-LEGO）系统在活线虫（线虫）。研究表明，红外辐射可诱导单个细胞基因表达，而不引起细胞损伤。在这里，我们报告IR-LEGO的应用程序的小鱼，青鳉（日本鳉鱼;青鳉）和斑马鱼（斑马鱼），和高等植物（拟南芥）。使用容易观察到的报告基因，我们成功地诱导基因在这些活生物体的各种组织中的表达。IR-LEGO有潜力成为广泛研究领域的有用工具，用于在小鱼和植物的局部组织中进行细胞/组织标记或靶向基因表达。
Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.