INHIBITION OF GROWTH OF HELA AND WI-38 CELLS BY DEHYDROEPIANDROSTERONE AND ITS REVERSAL BY RIBONUCLEOSIDES AND DEOXYRIBONUCLEOSIDES
INHIBITION OF GROWTH OF HELA AND WI-38 CELLS BY DEHYDROEPIANDROSTERONE AND ITS REVERSAL BY RIBONUCLEOSIDES AND DEOXYRIBONUCLEOSIDES
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DOI：
10.1016/0024-3205(86)90557-6
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发表时间：
1986-04-21
期刊：
影响因子：
6.1
通讯作者：
SCHWARTZ, AG
中科院分区：
文献类型：
--
作者：
DWORKIN, CR;GORMAN, SD;SCHWARTZ, AG
脱氢表雄酮（DHEA）是一种肾上腺甾体激素，是哺乳动物葡萄糖-6-磷酸脱氢酶（G6 PDH）的有效抑制剂。DHEA对体外培养的两株HeLa细胞和WI-38细胞的生长均有抑制作用。其中一株HeLa细胞株TCRC-2为10.5倍。与其他两种细胞系一样对生长抑制敏感。HeLa TCRC-2细胞提取物中的G6 PDH活性也比其他细胞系的G6 PDH活性对DHEA抑制更敏感。向培养基中添加四种脱氧核糖核苷和四种核糖核苷的组合克服了HeLa TCRC-2系中的DHEA诱导的生长抑制。
Dehydroepiandrosterone (DHEA), an adrenal steroid of no known biological function, is a potent inhibitor of mammalian glucose-6-phosphate dehydrogenase (G6PDH). DHEA inhibited the growth of two strains of HeLa and WI-38 cells in culture. One of the HeLa strains, TCRC-2, was about 10.times. as sensitive to growth inhibition as the two other cell lines. The G6PDH activity in cell extracts of HeLa TCRC-2 was also much more sensitive to DHEA inhibition than the G6PDH activities of the other cell lines. The addition of a combination of four deoxyribonucleosides and four ribonucleosides to the culture medium overcame the DHEA-induced growth inhibition in the HeLa TCRC-2 line.