Inorganic mercury changes the fate of murine CNS stem cells
Inorganic mercury changes the fate of murine CNS stem cells
复制标题
DOI：
10.1096/fj.02-0491fje
复制
发表时间：
2003-03-01
期刊：
影响因子：
4.8
通讯作者：
La Porta, CAM
中科院分区：
文献类型：
--
作者：
Cedrola, S;Guzzi, G;La Porta, CAM
从胚胎和成年小鼠的中枢神经系统分离的干细胞可以通过依赖于暴露于适当的表观遗传信号的不同分化模式的激活来产生神经元和神经胶质。另一方面，环境条件可能影响这些细胞的增殖、迁移和分化。我们在这里报告，第一次，无机汞影响成人神经干细胞（ANSC）的增殖和分化能力。事实上，无机汞会增加ASNC的细胞凋亡。此外，在干细胞衍生的星形胶质细胞中，出现高水平的70 kDa热休克蛋白（HSP-70），而GTP-β-微管蛋白活性水平显著降低。有趣的是，当诱导分化时，无机汞改变星形胶质细胞的形态学特性，而神经元数量减少。这些结果表明，无机汞在ANSC衍生的神经元群体中产生毒性，并影响神经胶质衍生群体的生物学特性。
Stem cells isolated from the central nervous system of both embryonic and adult mice can generate neurons and glia through the activation of different patterns of differentiation in dependence of exposure to appropriate epigenetic signals. On the other hand, environmental conditions might affect the proliferation, migration, and differentiation of these cells. We report here, for the first time, that inorganic mercury affects the proliferative and differentiative capacity of adult neuronal stem cells (ANSCs). Actually, inorganic mercury increases apoptosis in ASNC. Furthermore, in stem cell-derived astrocytes, high levels of the 70 kDa heat shock protein (HSP-70) occur, while the levels of GTP-beta-tubulin activity dramatically decrease. Interestingly, when induced to differentiate, inorganic mercury modifies morphological proprieties of astrocytes, while the neuron population is reduced. These results demonstrate that inorganic mercury produces toxicity in the ANSC-derived neuronal population and affects the biological properties of the glial-derived population.