Biocompatible chitosan-DNAzyme nanoparticle exhibits enhanced biological activity
Biocompatible chitosan-DNAzyme nanoparticle exhibits enhanced biological activity
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DOI：
10.1080/02652040802033673
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发表时间：
2008-01-01
影响因子：
3.9
通讯作者：
Choong, Peter F. M.
中科院分区：
文献类型：
--
作者：
Dass, Crispin R.;Friedhuber, Anna M.;Choong, Peter F. M.
DNA酶是能够特异性催化靶mRNA的寡核苷酸。迄今为止，还没有开发出DNA酶的递送载体。壳聚糖是一种在自然界中大量存在的生物材料。发现由壳聚糖配制的生物相容性c-jun（一种致癌基因）DNAzyme纳米颗粒对骨肉瘤（骨癌）细胞更具活性，诱导这些细胞中的凋亡细胞死亡。该制剂在血清中稳定一周，在室温下稳定一个月。临床上，用壳聚糖纳米生物技术敲低c-jun基因表达可能会改善骨肿瘤的治疗效果。
DNAzymes are oligonucleotides capable of specific catalysis of target mRNA. To date, a delivery vehicle for DNAzymes has not been developed. Chitosan is a biomaterial obtained abundantly in nature. A biocompatible c-jun (an oncogene) DNAzyme nanoparticle formulated from chitosan was found to be more active against osteosarcoma (bone cancer) cells, inducing apoptotic cell death in these cells. The formulation was stable in serum for a week and at room temperature for a month. Clinically, knockdown of c-jun gene expression with chitosan nanobiotechnology may improve treatment outcome for tumours growing in bone.