Identification and regulation of cold-inducible factors of Bordetella bronchiseptica.
Identification and regulation of cold-inducible factors of Bordetella bronchiseptica.
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DOI：
10.1099/mic.0.27785-0
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发表时间：
2005-06
期刊：
影响因子：
1.5
通讯作者：
D. Stübs;T. Fuchs;B. Schneider;A. Bosserhoff;R. Gross
中科院分区：
文献类型：
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作者：
D. Stübs;T. Fuchs;B. Schneider;A. Bosserhoff;R. Gross
细菌冷休克蛋白（CSPs）的表达在低温下被高度诱导，一些CSPs是细胞在低温下恢复生长所必需的。支气管杆菌编码5种csp（命名为CspA至CspE），与大肠杆菌的CspA具有显著的氨基酸同源性。与大肠杆菌相反，单个csp基因（cspB）的插入敲除强烈地影响分枝杆菌的生长，而不受温度的影响。在三个csp基因（cspA, cspB, cspC）的情况下，可以检测到不止一个特定的转录物。冷休克后，cspA、cspB和cspC转录本的净数量明显增加，而cspD和cspE的净数量没有明显增加。在其他胁迫条件下，包括翻译抑制剂、热休克、渗透胁迫和固定期的营养剥夺，表明csp基因也对这些条件有反应。所有冷休克基因的编码区之前都有一个很长的非翻译上游区域（5'-UTR）。以cspB基因为例，该区域的部分缺失导致截断的转录物的翻译显著减少，表明5'-UTR在翻译控制中起作用。通过2D-PAGE和质谱表征对冷激刺激进行了研究，鉴定出了额外的冷诱导蛋白（cip）。有趣的是，两个冷休克基因（cspC和cspD）被发现受到博德氏菌毒力基因的主要转录调控因子BvgAS系统的负调控。此外，CspB轻微过表达对百日咳杆菌腺苷酸环化酶毒素CyaA的转录有负面影响，而其他CSPs则没有，这表明csp介导的应激反应刺激与博德特拉毒力调节之间存在交叉作用。
The expression of bacterial cold-shock proteins (CSPs) is highly induced in response to cold shock, and some CSPs are essential for cells to resume growth at low temperature. Bordetella bronchiseptica encodes five CSPs (named CspA to CspE) with significant amino acid homology to CspA of Escherichia coli. In contrast to E. coli, the insertional knock-out of a single csp gene (cspB) strongly affected growth of B. bronchiseptica independent of temperature. In the case of three of the csp genes (cspA, cspB, cspC) more than one specific transcript could be detected. The net amount of cspA, cspB and cspC transcripts increased strongly after cold shock, while no such effect could be observed for cspD and cspE. The exposure to other stress conditions, including translation inhibitors, heat shock, osmotic stress and nutrient deprivation in the stationary phase, indicated that the csp genes are also responsive to these conditions. The coding regions of all of the cold-shock genes are preceded by a long non-translated upstream region (5'-UTR). In the case of the cspB gene, a deletion of parts of this region led to a significant reduction of translation of the resulting truncated transcript, indicating a role of the 5'-UTR in translational control. The cold-shock stimulon was investigated by 2D-PAGE and mass spectrometric characterization, leading to the identification of additional cold-inducible proteins (CIPs). Interestingly, two cold-shock genes (cspC and cspD) were found to be under the negative control of the BvgAS system, the main transcriptional regulator of Bordetella virulence genes. Moreover, a negative effect of slight overexpression of CspB, but not of the other CSPs, on the transcription of the adenylate cyclase toxin CyaA of Bordetella pertussis was observed, suggesting cross-talk between the CSP-mediated stress response stimulon and the Bordetella virulence regulon.