Development of an Attenuated Total Reflection Based Fiber-Optic Sensor for Real-time Sensing of Biofilm Formation
Development of an Attenuated Total Reflection Based Fiber-Optic Sensor for Real-time Sensing of Biofilm Formation
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DOI：
10.2116/analsci.33.883
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发表时间：
2017-08
影响因子：
1.6
通讯作者：
Tatsuya Orii;Takuya Okazaki;N. Hata;K. Sugawara;F. Rahman;H. Kuramitz
中科院分区：
文献类型：
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作者：
Tatsuya Orii;Takuya Okazaki;N. Hata;K. Sugawara;F. Rahman;H. Kuramitz
开发了一种能够实时监测水中生物膜形成的光纤传感器。通过去除多模光纤的包层以暴露纤芯，可以轻松制造该传感器。传感作用基于全内反射过程中倏逝波穿透暴露光纤芯表面上形成的生物膜。所提出的装置可用于使用白光源和光谱检测器来分析宽波长范围内的透射率响应。观察了透射率相对于纤维芯表面上生物膜形成的变化。这项研究的结果表明，传感器检测在近红外波长处比在可见光波长处具有更好的灵敏度。此外，该传感器的灵敏度可以通过静电相互作用对核心表面进行表面修饰来控制，涉及硅烷偶联层、聚阴离子和聚阳离子。所开发的传感器已成功应用于评估商业生物膜抑制剂的有效性。
A fiber-optic sensor capable of real-time monitoring of biofilm formation in water was developed. The sensor can be easily fabricated by removing the cladding of a multimode fiber optic to expose the core. The sensing action is based on the penetration of an evanescent wave through a biofilm formed on the surface of the exposed fiber core during total internal reflection. The proposed setup can be used to analyze the transmittance response over a wide wavelength range using a white-light source and a spectroscopy detector. The change in transmittance with respect to the biofilm formation on the fiber core surface was observed. The findings from this study showed that the sensor detection had better sensitivity at near-infrared wavelengths than at visible-light wavelengths. Moreover, the sensitivity of this sensor could be controlled by surface modifications of the core surface through electrostatic interactions, involving a silane coupling layer, polyanions, and polycations. The developed sensor was successfully applied to evaluating of the effectiveness of a commercial biofilm inhibitor.