Differential Regulation of Soybean Seed Storage Protein Gene Promoter-GUS Fusions by Exogenously Applied Methionine in Transgenic Arabidopsis thaliana
Differential Regulation of Soybean Seed Storage Protein Gene Promoter-GUS Fusions by Exogenously Applied Methionine in Transgenic Arabidopsis thaliana
复制标题
外源蛋氨酸对转基因拟南芥大豆种子贮藏蛋白基因启动子-GUS融合体的差异调控
DOI：
10.1093/oxfordjournals.pcp.a078678
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发表时间：
1994
影响因子：
4.9
通讯作者：
S. Naito
中科院分区：
文献类型：
--
作者：
M. Hirai;T. Fujiwara;K. Goto;Y. Komeda;M. Chino;S. Naito
已证明，在体外大豆子叶培养物中，外源施用的 L-蛋氨酸会下调编码 β-伴大豆球蛋白（大豆 7S 种子储存蛋白（Glycine max[L.] Merr.））β 亚基的基因的表达，而编码 thea' 亚基的基因的表达不受影响。我们构建了转基因拟南芥(L.) Heynh。携带受 thea' 或 β 亚基基因启动子区域控制的 β-葡萄糖醛酸酶 (GUS) 报告基因。未成熟长角果的体外培养系统。拟南芥是为向未成熟种子外源施用 L-蛋氨酸而开发的。 L-蛋氨酸的应用下调了fi亚基基因启动子-GUS融合体的表达，并且这种反应是可逆的。作为应用 L-蛋氨酸的替代方法，A.拟南芥植物生长在岩棉上，并用补充 L-蛋氨酸的培养基进行灌溉。由β亚基基因启动子驱动的GUS活性被L-蛋氨酸抑制，抑制程度取决于培养基中L-蛋氨酸的浓度。另一方面，thea'亚基基因启动子-GUS融合体则没有表现出这样的反应。这些结果表明A.拟南芥有一个在转录水平上通过 L-蛋氨酸差异调节 β-伴大豆球蛋白基因表达的系统。这项工作建立了一个系统来对植物对营养刺激的反应进行基因分析。
Expression of the gene encoding the β subunit of β-conglycinin, the 7S seed storage protein of soybean (Glycine max[L.] Merr.), has been shown to be down-regulated by exogenously applied L-methionine in in vitro soybean cotyledon cultures, whereas expression of the gene encoding thea′ subunit is not affected. We constructed transgenicArabidopsis thaliana(L.) Heynh. carrying the β-glucuronidase (GUS) reporter gene under the control of the promoter regions from either thea′ or β subunit gene. An in vitro culture system of immature siliques ofA. thalianawas developed for exogenous application of L-methionine to immature seeds. Expression of the fi subunit gene promoter-GUS fusion was down-regulated by the application of L-methionine, and this response was reversible. As an alternative way to apply L-methionine,A. thalianaplants were grown on rockwool and irrigated with L-methionine-supplemented medium. GUS activity driven by the β subunit gene promoter was repressed by L-methionine, and the degree of repression depended on the concentration of L-methionine in the medium. On the other hand, thea′ subunit gene promoter-GUS fusion did not show such a response. These results indicate thatA. thalianahas a system to differentially regulate the expression of the β-conglycinin genes by L-methionine at the level of transcription. This work establishes a system to genetically analyze plants′ responses to nutritional stimuli.