Toxicity of Stigmasterol Isolated from Crofton Weed, Eupatorium adenophorum Spreng. Against a Rabbit Ear Mite, Psoroptes cuniculi
Toxicity of Stigmasterol Isolated from Crofton Weed, Eupatorium adenophorum Spreng. Against a Rabbit Ear Mite, Psoroptes cuniculi
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DOI：
10.17582/journal.pjz/2017.49.4.1197.1200
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发表时间：
2017-08-01
影响因子：
0.6
通讯作者：
Wang, Gang
中科院分区：
文献类型：
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作者：
Nong, Xiang;Yang, Yao-jun;Wang, Gang
为了开发新型杀虫剂，本研究为从紫茎泽兰中开发可释放到环境中的无毒、无污染农药豆甾醇奠定了基础。对豆甾醇的体外毒性试验表明，在0.5%浓度下，豆甾醇具有毒性。在此条件下处理，12h内的死亡率为16.67%，24 h内的死亡率为23.33%，豆甾醇的杀虫活性弱于紫茎泽兰中的苷元化合物。通过初步分离。然而，在实验中，我们发现了一个有趣的现象，那就是用豆甾醇处理的螨类的死亡状态与用氰戊菊酯处理的阳性对照组的螨类相似。此外，豆甾醇处理的尘螨的死亡状态与阴性对照组不同。这一结果证实了豆甾醇的杀虫活性。我们对豆甾醇的杀虫分子机理进行了深入的研究，为开发豆甾醇作为无毒、无污染的农药进入环境奠定了基础。
In order to develop a new type of insecticide, this study lays foundation for the development of stigmasterol from the Eupatorium adenophorum Sprengas nontoxic and pollution-free pesticides that can be released to the environment. The toxicity test of stigmasterol against Psoroptes cuniculi in vitro showed that stigmasterol was toxic under 0.5% concentration. Treatment under this condition also showed mite mortality of 16.67% within 12 h and 23.33% within 24 h. The insecticidal activity of stigmasterol was weaker than that of ageraphorone compound obtained from E. adenophorum Spreng. Through preliminary separation. However, in the experiments, we found an interesting phenomenon, that is, the dead state of mites treated with stigmasterol was similar to that of mites of the positive control group treated with fenvalerate. Furthermore, the dead state of mites treated with stigmasterol was different from that of mites of the negative control group. This result confirmed the insecticidal activity of stigmasterol. We conducted a thorough research on the insecticidal molecular mechanism of stigmasterol to lay the foundation for the development of stigmasterol as nontoxic and pollution-free pesticides that can be introduced into the environment.