Measurement of local electric field in microdevices for low-voltage electroporation of adherent cells
Measurement of local electric field in microdevices for low-voltage electroporation of adherent cells
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DOI：
10.1007/s00542-013-1797-9
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发表时间：
2014-02
期刊：
影响因子：
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通讯作者：
H. Shintaku;Kazumi Hakamada;H. Fujimoto;Takeshi Nagata;J. Miyake;S. Kawano
中科院分区：
文献类型：
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作者：
H. Shintaku;Kazumi Hakamada;H. Fujimoto;Takeshi Nagata;J. Miyake;S. Kawano
在这项研究中，我们提出了测量的局部电场的微型装置设计的电穿孔的贴壁细胞。微器件主要由具有透明导电层和绝缘层的盖玻片组成。绝缘层有小的圆柱形孔，这些孔集中场线以降低电穿孔所需的电压。我们估计的局部电场在细胞通过分析离子电流的基础上一个简单的等效电路模型，并研究了电场强度和电穿孔效率之间的相关性。我们准备了各种电极矩阵设计，直径范围为5至10 μm，相邻电极之间的中心距范围为20至75 μm，以进行系统和统计研究。此外，我们讨论了效率的电极设计方面的领域聚焦的程度，光学观测的适用性，和定位细胞的电极上的概率。
In this study, we present the measurement of the local electric field in a microdevice designed for electroporation of adherent cells. The microdevice mainly consists of a coverslip that has a transparent conductive layer and an insulating layer. The insulating layer has small cylindrical holes that focus the field lines to reduce the voltage required for electroporation. We estimated the local electric field at the cells by analyzing the ionic current based on a simple equivalent circuit model and investigated the correlation between the field strength and the efficiency of electroporation. We prepared various designs with matrices of electrodes with diameters ranging from 5 to 10 μm and center-to-center distances between adjacent electrodes ranging from 20 to 75 μm to perform systematic and statistical investigations. Furthermore, we discussed the efficiency of the electrode design in terms of the degree of field focusing, the applicability of optical observations, and the probability of positioning cells on the electrodes.