Studies on the relationship between 5' leader sequences and initiation of translation of adenovirus 2 and simian virus 40 late mRNAs.
Studies on the relationship between 5' leader sequences and initiation of translation of adenovirus 2 and simian virus 40 late mRNAs.
复制标题
腺病毒2型和猿猴病毒40型晚期mRNA 5前导序列与翻译起始关系的研究。
DOI：
10.1016/0042-6822(80)90450-x
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Hsu,MT
中科院分区：
文献类型：
--
作者：
Wolgemuth,DJ;Yu,HY;Hsu,MT
The question of whether or not 5′ leader sequences might confer characteristic translational properties to the mRNAs to which they are spliced was studied using two complementary experimental situations with respect to the composition of mRNA molecules. Late in adenovirus type 2 (Ad-2) infection of human cells, a common tripartite 5′ leader sequence is spliced to mRNA sequences coding for at least 13 different late viral proteins. This results in different mRNAs with identical 5′ leader sequences. In the second experimental system, identical coding sequences are spliced to different 5′ leader sequences with different caps. Wild-type SV40 VP1 mRNA (16 S) contains a 5′ leader sequence coded for at position72–76on the SV40 genome; in the SV40 mutant dl-808 part of the sequences in this region, including the sequence coding for cap, is deleted. The relative ability of these mRNAs to direct incorporation of radioactive amino acids into their respective polypeptide products was assayedin vivounder conditions of hypertonic initiation block (HIB) or in the presence of low concentrations of cycloheximide. Messenger RNAs with identical 5′ leader sequences were observed to exhibit very different responses in both experimental situations. Conversely, similar responses to HIB were observed for VP1 mRNAs with different 5′ leader sequences. It thus appears that the presence of specific sequences per se between the 5′ cap and the initiation codon does not alone confer characteristic initiation properties as measured indirectly by perturbation of thein vivoenvironment.
