Eukaryotic viruses in the fecal virome at the onset of type 1 diabetes: A study from four geographically distant African and Asian countries
Eukaryotic viruses in the fecal virome at the onset of type 1 diabetes: A study from four geographically distant African and Asian countries
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DOI：
10.1111/pedi.13207
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发表时间：
2021-04-07
影响因子：
3.4
通讯作者：
Abdullah, Mohammed Ahmed
中科院分区：
文献类型：
--
作者：
Cinek, Ondrej;Kramna, Lenka;Abdullah, Mohammed Ahmed
目的1型糖尿病（T1 D）粪便病毒组的研究仅限于欧洲和美国人群。因此，我们试图描述粪便病毒组在儿童发病后T1 D和匹配的对照受试者从四个地理上遥远的非洲和亚洲countries.Methods粪便样本收集73名儿童和青少年T1 D发病后不久（阿塞拜疆19，约旦20，尼日利亚14，苏丹20）和105匹配的对照受试者相似的年龄和地点。对DNA和RNA病毒组进行宏基因组测序，病毒阳性定义为超过样品读数的0.001%。还使用实时PCR对选定的病毒进行定量。条件Logistic回归模型与真核病毒positivity.Results信号的387个不同的病毒物种被检测到，至少有一个真核病毒被检测到71%的情况下，65%的对照样品。观察到的真核病毒种或属在T1 D儿童和对照组之间的频率均无差异。每个样本中不同病毒属的总计数在病例之间存在提示性关联（1.45属）和对照（1.10属，OR 1.24，95%CI 0.98-1.57），一项计划外的亚组分析表明，在病例中内源性逆转录病毒信号的频率略高。（28.8%vs.8.6%，OR = 4.55，95%CI 1.72-12）。结论在来自四个遥远的非欧洲人群的粪便病毒组中未观察到与T1 D的明确和一致的关联。人类内源性逆转录病毒的临界关联的发现值得进一步探索。
Objectives Studies of the fecal virome in type 1 diabetes (T1D) have been limited to populations of Europe and the United States. We therefore sought to characterize the stool virome in children after onset of T1D and in matched control subjects from four geographically distant African and Asian countries.Methods Samples of stool were collected from 73 children and adolescents shortly after T1D onset (Azerbaijan 19, Jordan 20, Nigeria 14, Sudan 20) and 105 matched control subjects of similar age and locale. Metagenomic sequencing of the DNA and RNA virome was performed, and virus positivity was defined as more than 0.001% of reads of the sample. Selected viruses were also quantified using real-time PCR. Conditional logistic regression was used to model associations with eukaryotic virus positivity.Results Signals of 387 different viral species were detected; at least one eukaryotic virus was detected in 71% case and 65% control samples. Neither of observed eukaryotic virus species or genera differed in frequency between children with T1D and controls. There was a suggestive association of the total count of different viral genera per sample between cases (1.45 genera) and controls (1.10 genera, OR 1.24, 95%CI 0.98-1.57), and an unplanned subanalysis suggested marginally more frequent endogenous retrovirus signal in cases (in 28.8% vs. in 8.6% controls, OR = 4.55, 95%CI 1.72-12).Conclusions No clear and consistent association with T1D was observed in the fecal viromes from four distant non-European populations. The finding of borderline associations of human endogenous retroviruses merits further exploration.